Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Antibacterial Components of Levisticum officinale Koch against Multidrug-resistant Mycobacterium tuberculosis

Mon Oct 3 22:10:28 2022

(2020) Antibacterial Components of Levisticum officinale Koch against Multidrug-resistant Mycobacterium tuberculosis. Pharmaceutical Sciences. pp. 441-447. ISSN 1735-403X

Full text not available from this repository.

Official URL: http://apps.webofknowledge.com/InboundService.do?F...

Abstract

Background: A bioassay-guided fractionation technique was used to evaluate the active constituents of the perennial plant L. officinale W.D.J. Koch (Apiaceae) against multidrug resistant (MDR) Mycobacterium tuberculosis. Methods: Column chromatography was used to isolation of compounds from L. officinale and spectroscopic methods including 1D and 2D NMR (Nuclear magnetic resonance) and HRMS (high resolution mass spectrometry) were used to identification of the isolated compounds. Also, to evaluate antibacterial activity, minimum inhibitory concentration (MIC) was carried out by broth micro-dilution method. Finally, molecular docking (MD) was performed using the Schrodinger package to evaluate interactions between the active compounds and InhA protein. Results: Phytochemical analysis of the ethyl acetate extract of the plant roots led to isolation of bergapten (1), isogosferol (2), oxypeucedanin (3), oxypeucedanin hydrate (4), imperatorin (5), ferulic acid (6) and falcarindiol (7). Falcarindiol and oxypeucedanin indicated a moderate activity on MDR M. tuberculosis with MIC values of = 32 and 64 mu g/mL, respectively. Antibacterial activity of falcarindiol was also observed against S. aureus and methicillin-resistant S. aureus strains with the MIC values of 7.8 and 15.6 mu g/mL, respectively. The results of docking analysis showed a good affinity of oxypeucedanin (3) and falcarindiol (7) to InhA enzyme with docking score values of -7.764 and -7.703 kcal/mol, respectively. Conclusion: Finally, 7 compounds were isolated from L. officinale that compounds 2-6 report for the first time from this plant. On the basis of the molecular docking (MD) study, oxypeucedanin (3) and falcarindiol (7) as active compounds against M. tuberculosis may be proposed as potential inhibitors of 2-trans-enoyl-ACP reductase (InhA), a key enzyme involved in the biosynthesis of the mycobacterial cell wall. Moreover, antibacterial activity of falcarindiol against methicillin-resistant S. aureus (MRSA) was remarkable.

Item Type: Article
Creators:
CreatorsEmail
Monsefesfahani, H.UNSPECIFIED
Farimani, M. M.UNSPECIFIED
Ebrahimi, S. N.UNSPECIFIED
Jung, J. H.UNSPECIFIED
Aliahmadi, A.UNSPECIFIED
Abbas-Mohammadi, M.UNSPECIFIED
Skropetas, D.UNSPECIFIED
Kazemian, H.UNSPECIFIED
Feizabadi, M.UNSPECIFIED
Miran, M.UNSPECIFIED
Keywords: Antibacterial activity Bioassay-guided fractionation Levisticum officinale Molecular docking Multidrug resistance Mycobacterium tuberculosis essential oil identification constituents polyynes strains docking Pharmacology & Pharmacy
Divisions:
Page Range: pp. 441-447
Journal or Publication Title: Pharmaceutical Sciences
Journal Index: ISI
Volume: 26
Number: 4
Identification Number: https://doi.org/10.34172/ps.2020.38
ISSN: 1735-403X
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3346

Actions (login required)

View Item View Item