Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Bioinformatics analysis of calcium-dependent protein kinase 4 (CDPK4) as Toxoplasma gondii vaccine target

Fri Aug 19 02:53:41 2022

(2021) Bioinformatics analysis of calcium-dependent protein kinase 4 (CDPK4) as Toxoplasma gondii vaccine target. Bmc Research Notes. p. 9.

Full text not available from this repository.

Official URL: http://apps.webofknowledge.com/InboundService.do?F...

Abstract

Objectives Toxoplasma gondii (T. gondii), an obligate intracellular apicomplexan parasite, could affect numerous warm-blooded animals, such as humans. Calcium-dependent protein kinases (CDPKs) are essential Ca2+ signaling mediators and participate in parasite host cell egress, outer membrane motility, invasion, and cell division. Results Several bioinformatics online servers were employed to analyze and predict the important properties of CDPK4 protein. The findings revealed that CDPK4 peptide has 1158 amino acid residues with average molecular weight (MW) of 126.331 KDa. The aliphatic index and GRAVY for this protein were estimated at 66.82 and - 0.650, respectively. The findings revealed that the CDPK4 protein comprised 30.14 and 34.97 alpha-helix, 59.84 and 53.54 random coils, and 10.02 and 11.49 extended strand with SOPMA and GOR4 tools, respectively. Ramachandran plot output showed 87.87, 8.40, and 3.73 of amino acid residues in the favored, allowed, and outlier regions, respectively. Also, several potential B and T-cell epitopes were predicted for CDPK4 protein through different bioinformatics tools. Also, antigenicity and allergenicity evaluation demonstrated that this protein has immunogenic and non-allergenic nature. This paper presents a basis for further studies, thereby provides a fundamental basis for the development of an effective vaccine against T. gondii infection.

Item Type: Article
Creators:
CreatorsEmail
Foroutan, M.UNSPECIFIED
Ghaffari, A. D.UNSPECIFIED
Soltani, S.UNSPECIFIED
Majidiani, H.UNSPECIFIED
Taghipour, A.UNSPECIFIED
Sabaghan, M.UNSPECIFIED
Keywords: Toxoplasma gondii Calcium-dependent protein kinase 4 Bioinformatics analysis In silico Vaccine Life Sciences & Biomedicine - Other Topics Science & Technology - Other Topics
Divisions:
Page Range: p. 9
Journal or Publication Title: Bmc Research Notes
Journal Index: ISI
Volume: 14
Number: 1
Identification Number: https://doi.org/10.1186/s13104-021-05467-1
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3316

Actions (login required)

View Item View Item