Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Multi-epitope vaccine against cystic echinococcosis using immunodominant epitopes from EgA31 and EgG1Y162 antigens

Mon Sep 26 22:58:21 2022

(2020) Multi-epitope vaccine against cystic echinococcosis using immunodominant epitopes from EgA31 and EgG1Y162 antigens. Informatics in Medicine Unlocked. ISSN 23529148 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Cystic echinococcosis (CE) is a near-cosmopolitan public health concern, especially in developing countries. Parasite ova shed via definitive host feces are responsible for livestock and occasionally human infection, leading to hydatid cysts. Immunization using proper antigens is a good immunoprophylactic strategy. This study was aimed to design a multi-epitope vaccine using EgA31 and EgG1Y162 antigens. Top high-ranked B-cell epitopes and major histocompatibility complex (MHC)-binding epitopes were predicted and selected to construct the vaccine model. Then, physico-chemical features, secondary and tertiary structures, refinement and validations were all evaluated using web servers for the multi-epitope vaccine construct. Finally, non-linear B-cell epitopes were determined for vaccine-antibody interactions. Moreover, the vaccine construct was subjected to disulfide engineering, molecular docking with human MHC-I and MHC-II molecules as well as codon adaptation and in silico cloning processes. In conclusion, this multimeric CE vaccine needs experimental and clinical confirmations to be considered as an actually immunogenic vaccine model. © 2020

Item Type: Article
Creators:
CreatorsEmail
Nourmohammadi, H.UNSPECIFIED
Javanmardi, E.UNSPECIFIED
Shams, M.UNSPECIFIED
Shamsinia, S.UNSPECIFIED
Nosrati, M. C.UNSPECIFIED
Yousefi, A.UNSPECIFIED
Nemati, T.UNSPECIFIED
Fatollahzadeh, M.UNSPECIFIED
Ghasemi, E.UNSPECIFIED
Kordi, B.UNSPECIFIED
Majidiani, H.UNSPECIFIED
Irannejad, H.UNSPECIFIED
Keywords: Echinococcus granulosus EgA31 EgG1Y162 Multi-epitope vaccine disulfide ega31 antigen egg1y162 antigen epitope major histocompatibility antigen class 1 major histocompatibility antigen class 2 parasite antigen unclassified drug vaccine Article B lymphocyte bioinformatics codon computer model epitope mapping major histocompatibility complex molecular cloning molecular docking nonhuman physical chemistry protein secondary structure protein tertiary structure T lymphocyte
Divisions:
Journal or Publication Title: Informatics in Medicine Unlocked
Journal Index: Scopus
Volume: 21
Identification Number: https://doi.org/10.1016/j.imu.2020.100464
ISSN: 23529148 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3308

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