Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Amelioration of cerebellar dysfunction in rats following postnatal ethanol exposure using low-intensity pulsed ultrasound

Tue Oct 4 01:27:19 2022

(2017) Amelioration of cerebellar dysfunction in rats following postnatal ethanol exposure using low-intensity pulsed ultrasound. Journal of Chemical Neuroanatomy. pp. 71-75. ISSN 0891-0618

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Abstract

Background: The neonatal development stage of the cerebellum in rats is equivalent to a human foetus in the third trimester of pregnancy. In this stage, cell proliferation, migration, differentiation, and synaptogenesis occur. Clinical and experimental findings have shown that ethanol exposure during brain development causes a variety of disruptions to the brain, including neurogenesis depression, delayed neuronal migration, changes in neurotransmitter synthesis, and neuronal depletion.During postnatal cerebellar development, neurons are more vulnerable to the destructive effects of ethanol. The effects of low-intensity pulsed ultrasound (LIPUS) on the number of cells and thickness of the cell layers within the cerebellar cortex were examined during the first two postnatal weeks in rats following postnatal ethanol exposure. Method: Postpartum rats were distributed randomly into six groups. Normal saline was injected intraperitoneally into control animals and ethanol (20) was injected into the intervention groups for three consecutive days. Intervention groups received LIPUS at different frequencies (3 or 5 MHz), after administration of ethanol. After transcardial perfusion, the rat's brain was removed, and a complete series of sagittal cerebellum sections were obtained by systematic random manner. Photomicrographs were made with Motic digital cameras and analysed using Nikon digital software. Results: The numbers of granular cells decreased in ethanol-treated rats compared to the control group. LIPUS, administered at (3 or 5 MHz), combined with ethanol administration resulted in a reduction of ethanol's effects. Using 5 MHz LIPUS resulted in significantly higher numbers of granular cells in the internal layer compared to the control rats. Using 3 or 5 MHz LIPUS alone resulted in a significant enhancement in the granular cells of the molecular layer. A significant reduction was seen in the thickness of the external granular layer in ethanol-treated rats. Conclusion: This study showed that exposure to LIPUS can affect the number of granular cells and thickness of the cell layer within the cerebellar cortex in neonatal rats. LIPUS also could attenuate ethanol toxicity effects on the cerebellum. (C) 2017 Elsevier B.V. All rights reserved.

Item Type: Article
Creators:
CreatorsEmail
Bolbanabad, H. M.UNSPECIFIED
Anvari, E.UNSPECIFIED
Rezai, M. J.UNSPECIFIED
Moayeri, A.UNSPECIFIED
Kaffashian, M. R.UNSPECIFIED
Keywords: Granular cells count Lipus Ethanol Rat cell-migration neuronal migration neuropathology alcoholism motility Biochemistry & Molecular Biology Neurosciences & Neurology
Divisions:
Page Range: pp. 71-75
Journal or Publication Title: Journal of Chemical Neuroanatomy
Journal Index: ISI
Volume: 81
Identification Number: https://doi.org/10.1016/j.jchemneu.2017.02.006
ISSN: 0891-0618
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/330

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