Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Combined use of platelet-rich plasma and adipose tissue-derived mesenchymal stem cells shows a synergistic effect in experimental spinal cord injury

Mon Jun 27 10:40:41 2022

(2020) Combined use of platelet-rich plasma and adipose tissue-derived mesenchymal stem cells shows a synergistic effect in experimental spinal cord injury. Journal of chemical neuroanatomy. p. 101870. ISSN 1873-6300 (Electronic) 0891-0618 (Linking)

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Official URL: https://www.ncbi.nlm.nih.gov/pubmed/33038437

Abstract

Spinal cord injury (SCI) as a crippling disability causes tissue degeneration via neuron loss and fiber disruption. Some researchers have tried to reverse or minimize these changes. Platelet-rich plasma (PRP) is a biological product derived from peripheral blood containing a variety of growth factors. PRP has been extensively used in regenerative medicine. On the other hand, via secreting neuroprotective growth factors, mesenchymal stem cells (MSCs) have shown a promising potential in repairing central nervous system deficits. This study investigated the therapeutic effect of the combined use of MSCs and PRP in a rat model of SCI. We used real time-PCR method for evaluation of Bcl-2, Bax and caspase 3 expressions, TUNEL test for apoptotic cell death assessment, and neurofilament NF200 immunohistochemistry for examination of axonal regeneration. The results showed that co-treatment with MSCs and PRP efficiently alleviated the evaluated categories. Significant differences were observed in expression of Bcl-2 and caspase3, but not Bax, apoptotic index and the number of NF200 positive axons (for all P </= 0.01) between co-treatment animals compared with those treated with only MSCs or PRP. In conclusion, this study showed that combination of MSCs and PRP synergistically promotes their therapeutic effects in the SCI.

Item Type: Article
Creators:
CreatorsEmail
Salarinia, R.UNSPECIFIED
Hosseini, M.UNSPECIFIED
Mohamadi, Y.UNSPECIFIED
Ghorbani, A.UNSPECIFIED
Alamdari, D. H.UNSPECIFIED
Mafinezhad, A.UNSPECIFIED
Sadeghnia, H.UNSPECIFIED
Keywords: Combined therapy Mesenchymal stem cells Platelet-rich plasma Spinal cord injury
Divisions:
Page Range: p. 101870
Journal or Publication Title: Journal of chemical neuroanatomy
Journal Index: Pubmed
Volume: 110
Identification Number: https://doi.org/10.1016/j.jchemneu.2020.101870
ISSN: 1873-6300 (Electronic) 0891-0618 (Linking)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3260

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