Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Effects of Different Concentrations of Reversine on Plasticity of Mesenchymal Stem Cells

Sat Dec 10 02:40:36 2022

(2020) Effects of Different Concentrations of Reversine on Plasticity of Mesenchymal Stem Cells. Indian Journal of Clinical Biochemistry. pp. 188-196. ISSN 0970-1915

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Abstract

Dedifferentiation can be induced by small molecules. One of these small molecules used in this study in order to increase the plasticity of differentiation of stem cells was reversine. The objective of present study was to investigate the effect of different concentrations of reversine on the plasticity of ovine fetal bone-marrow mesenchymal stem cells (BM-MSCs). BM-MSCs were extracted from ovine fetal and cultured. Passaged-3 cells were evaluated for their differentiation potential into osteocytes and adipocytes cells. In the present study, BM-MSCs were culture plated in the presence of 0, 300, 600, 900 and 1200 nM of reversine. The number of viable cells was determined by MTT test after addition of different concentrations of reversine. Furthermore, expression of the nanog gene was evaluated. The culture without reversine was taken as the control group. Expression of nanog was analysed by immunocytochemistry. Multi-lineage differentiation showed that the BM-MSCs could be differentiated into adipose cells and osteocytes. Our results indicated that the addition of 1200 nM of reversine to medium significantly decreased overall proliferation compared to the other treatment groups (p > 0.05). Real-time PCR analysis showed that after addition of 600 nM of reversine significantly increased nanog expression compared to other treatments. All treatments received reversine were seen to be relative expression of nanog. Our findings confirm that low concentrations reversine increases the plasticity of ovine BM-MSCs.

Item Type: Article
Creators:
CreatorsEmail
Soltani, L.UNSPECIFIED
Rahmani, H. R.UNSPECIFIED
Joupari, M. D.UNSPECIFIED
Ghaneialvar, H.UNSPECIFIED
Mahdavi, A. H.UNSPECIFIED
Shamsara, M.UNSPECIFIED
Keywords: Reversine Ovine fetal Mesenchymal stem cells Proliferation Plasticity lineage-committed cells progenitor-like cells adipogenic differentiation gene-expression in-vitro dedifferentiation proliferation fibroblasts increases Biochemistry & Molecular Biology
Divisions:
Page Range: pp. 188-196
Journal or Publication Title: Indian Journal of Clinical Biochemistry
Journal Index: ISI
Volume: 35
Number: 2
Identification Number: https://doi.org/10.1007/s12291-018-0800-8
ISSN: 0970-1915
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3211

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