Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Effects of sub-inhibitory concentrations of antibiotics and oxidative stress on the expression of type II toxin-antitoxin system genes in Klebsiella pneumoniae

Thu Aug 11 15:10:37 2022

(2020) Effects of sub-inhibitory concentrations of antibiotics and oxidative stress on the expression of type II toxin-antitoxin system genes in Klebsiella pneumoniae. Journal of Global Antimicrobial Resistance. pp. 51-56. ISSN 2213-7165

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Abstract

Objectives: Sub-inhibitory concentrations (sub-MICs) of antibiotics reflect the conditions that bacteria encounter in tissues and the natural environment. Sub-MICs of antibiotics can induce stress and alter the expression of different bacterial genes. Bacteria react to stress conditions using different mechanisms, one of which is the toxin-antitoxin (TA) system. This study investigated the expression of the TA system genes under oxidative and antibiotic stresses in Klebsiella pneumoniae (K. pneumoniae). Methods: To determine the effects of sub-MICs of gentamicin, nalidixic acid, ceftazidime, and certain concentrations of H2O2 on bacterial survival and growth, colony forming units were quantitated and turbidity was assessed following the treatment of K. pneumoniae with 1/2 MICs of antibiotics and 5 mM H2O2 at different time intervals. The expression of TA system genes in K. pneumoniae was evaluated 1 h after treatment using the quantitative real-time PCR (qRT-PCR) method. Results: The results revealed reduced K. pneumoniae growth in the presence of sub-MICs of antibiotics and 5 mM H2O2 compared to the control. Furthermore, according to the results of the qRT-PCR assay, only the presence of gentamicin could increase the expression of TA system genes. Conclusion: Although the exact role of the TA systems in response to stress is still unclear, this study provided information on the effect of the type II TA systems under oxidative and antibiotic stress conditions. (C) 2019 International Society for Antimicrobial Chemotherapy. Published by Elsevier Ltd.

Item Type: Article
Creators:
CreatorsEmail
Narimisa, N.UNSPECIFIED
Amraei, F.UNSPECIFIED
Kalani, B. S.UNSPECIFIED
Mohammadzadeh, R.UNSPECIFIED
Jazi, F. M.UNSPECIFIED
Keywords: Sub-inhibitory antibiotic concentrations Oxidative stress Klebsiella pneumonia Toxin-antitoxin systems Real-time PCR pseudomonas-aeruginosa protease lon epidemiology virulence bacteria loci Infectious Diseases Pharmacology & Pharmacy
Divisions:
Page Range: pp. 51-56
Journal or Publication Title: Journal of Global Antimicrobial Resistance
Journal Index: ISI
Volume: 21
Identification Number: https://doi.org/10.1016/j.jgar.2019.09.005
ISSN: 2213-7165
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3209

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