Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Type II toxin/antitoxin system genes expression in persister cells of Klebsiella pneumoniae

Thu Aug 11 16:42:36 2022

(2020) Type II toxin/antitoxin system genes expression in persister cells of Klebsiella pneumoniae. Reviews in Medical Microbiology. pp. 215-220. ISSN 0954-139X

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Abstract

Objective: Persistence is a subpopulation of bacteria that can tolerate high doses of antibiotics by undergoing a period of dormancy. This study aimed to investigate the hypothetical role of toxin/antitoxin systems in persister cell formation of Klebsiella pneumoniae ATCC 13883 by evaluating toxins of type II toxin/antitoxin systems expression. Methods: To determine the number of surviving cells, bacterial colonies were counted at 1, 3, 5, 7, and 24 h after treated with a lethal dose of kanamycin. The role of toxin/antitoxin systems in persister cell formation was then determined by toxins of type II toxin/antitoxin systems (relE1, relE2, hipA, vapC, and doc) expression levels using real-time PCR. Results: Our results showed the viability of persister cells after 5 h. The results of relative real-time PCR showed higher levels of toxin gene expression due to stress conditions, suggesting the possible role of toxin/antitoxin systems in persister cell formation and antibiotic tolerance. Conclusion: Considering the importance of persistence and the tolerance to antibiotics, further studies on persister cell formation and related genes such as the toxin/antitoxin system genes might help us to identify the precise mechanisms leading to persister cell formation. Copyright (C) 2020 Wolters Kluwer Health, Inc. All rights reserved.

Item Type: Article
Creators:
CreatorsEmail
Narimisa, N.UNSPECIFIED
Kalani, B. S.UNSPECIFIED
Amraei, F.UNSPECIFIED
Mohammadzadeh, R.UNSPECIFIED
Mirkalantari, S.UNSPECIFIED
Razavi, S.UNSPECIFIED
Jazi, F. M.UNSPECIFIED
Keywords: antibiotics tolerance Klebsiella pneumonia persister cell real-time PCR toxin antitoxin system toxin-antitoxin systems molecular-mechanisms hipa frequency tolerance survival dormancy Microbiology
Divisions:
Page Range: pp. 215-220
Journal or Publication Title: Reviews in Medical Microbiology
Journal Index: ISI
Volume: 31
Number: 4
Identification Number: https://doi.org/10.1097/mrm.0000000000000232
ISSN: 0954-139X
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3186

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