Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Immunomodulatory Effects of Calcitriol through DNA Methylation Alteration of FOXP3 in the CD4(+) T Cells of Mice

Mon Sep 26 12:43:18 2022

(2020) Immunomodulatory Effects of Calcitriol through DNA Methylation Alteration of FOXP3 in the CD4(+) T Cells of Mice. Iranian Journal of Allergy Asthma and Immunology. 509-+. ISSN 1735-1502

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Abstract

Vitamin D plays a variety of physiological functions, such as regulating mineral homeostasis. More recently, it has emerged as an immunomodulator player, affecting several types of immune cells, such as regulatory T (Treg) cells. It has been reported that vitamin D exerts some mediatory effects through an epigenetic mechanism. In this study, the impacts of calcitriol, the active form of vitamin D, on the methylation of the conserved non-coding sequence 2 (CNS2) region of the forkhead box P3 (FOXP3) gene promoter, were evaluated. Fourteen C57BL/6 mice were recruited in this study and divided into two intervention and control groups. The CD4(+) T cells were isolated from mice splenocytes. The expression of FOXP3, IL-10, and transforming growth factor-beta (TGF-beta 1) genes were relatively quantified by real-time PCR technique, and the DNA methylation percentage of every CpG site in the CNS2 region was measured individually by bisulfite-sequencing PCR. Vitamin D Intervention could significantly (p<0.05) increase the expression of FOXP3, IL-10, and TGF-beta 1 genes in the CD4(+) T cells of mice comparing with the control group. Meanwhile, methylation of the CNS2 region of FOXP3 promoter was significantly decreased in three of ten CpG sites in the vitamin D group compared to the control group. The results of this study showed that vitamin D can engage the methylation process to induce FOXP3 gene expression and probably Treg cytokines profile. Further researches are needed to discover the precise epigenetic mechanisms by which vitamin D modulates the immune system.

Item Type: Article
Creators:
CreatorsEmail
Oraei, M.UNSPECIFIED
Bitarafan, S.UNSPECIFIED
Mesbah-Namin, S. A.UNSPECIFIED
Noori-Zadeh, A.UNSPECIFIED
Mansouri, F.UNSPECIFIED
Parastouei, K.UNSPECIFIED
Anissian, A.UNSPECIFIED
Yekaninejad, M. S.UNSPECIFIED
Hajizadeh, M.UNSPECIFIED
Saboor-Yaraghi, A. A.UNSPECIFIED
Keywords: Calcitriol FOXP3 gene Methylation Regulatory T-lymphocytes Vitamin D vitamin-d gene-expression genome-wide promotes generation cytokines elements stat3 Allergy Immunology
Divisions:
Page Range: 509-+
Journal or Publication Title: Iranian Journal of Allergy Asthma and Immunology
Journal Index: ISI
Volume: 19
Number: 5
Identification Number: https://doi.org/10.18502/ijaai.v19i5.4466
ISSN: 1735-1502
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3185

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