Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Evaluating the clinical application of nested PCR in the diagnosis offungal infections

Mon Aug 15 11:45:42 2022

(2020) Evaluating the clinical application of nested PCR in the diagnosis offungal infections. New Zealand Journal of Medical Laboratory Science. pp. 115-118. ISSN 11710195 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Background: Molecular techniques are appealing for the detection of human pathogens, but the detection offungal pathogens is particularly challenging. Fungi have cell walls that impede the efficient lysis of organisms and liberation of DNA, which can lead to false-negative results. Conversely, some human pathogens are ubiquitous environmental saprophytes that can contaminate PCR reagents and cause false-positive results. Aims and methods: In a cross-sectional study in Tehran, Iran from January 2017 to August 2018 we aimed to evaluate the incidence offungal infections in 420 specimens from patients suspected offungal disease and determined the clinical application of nested PCR for the diagnosis of fungal infections. Direct examination with 10 potassium hydroxide, culture on mycological media and nested polymerase chain reaction (PCR) by amplifying the conserved sequences of internal transcribed spacer 1 (ITS1) ribosomal DNA were performed in this study. Results: 79 (18.8 ) out of 420 specimens were positive by direct examination, including 64 (81) dermatophytes and 15(19 ) Aspergillus spp. 52 (12.3) had positive culture including 40 (76.9 ) dermatophytes and 12 (23.1) Aspergillus spp. 76/79 isolates were detected by nested-PCR (96.2). Conclusions: Our results showed that the incidence of fungal infections in the study population was relatively high and dermatophytes were the most common cause of fungal infections. Nested PCR had a high sensitivity and may be useful as a reliable screening method, especially in immunosuppressed and immunocompromised patients. © 2020 The authors.

Item Type: Article
Creators:
CreatorsEmail
Sheikhi, N.UNSPECIFIED
Rafat, Z.UNSPECIFIED
Abad, S. Z.UNSPECIFIED
Ahmadi, S. M.UNSPECIFIED
Azizian, R.UNSPECIFIED
Hamidi, M.UNSPECIFIED
Keywords: Aspergillus Culture Dermatophytes Direct smear Nested PCR
Divisions:
Page Range: pp. 115-118
Journal or Publication Title: New Zealand Journal of Medical Laboratory Science
Journal Index: Scopus
Volume: 74
Number: 2
ISSN: 11710195 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3140

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