Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Biofilm establishment, biofilm persister cell formation, and relative gene expression analysis of type II toxin-antitoxin system in Klebsiella pneumoniae

Thu Aug 11 17:16:45 2022

(2020) Biofilm establishment, biofilm persister cell formation, and relative gene expression analysis of type II toxin-antitoxin system in Klebsiella pneumoniae. Gene Reports. ISSN 24520144 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

The biofilms forming ability in Klebsiella pneumoniae isolates leads to treatment failure and chronic infections through protecting bacteria against antibiotics. Persister cells are a small fraction of the bacterial population that survives by entry into dormancy state after treatment with high dose antibiotics and are mainly responsible for a high level of biofilm-mediated tolerance to antibiotics. Toxin-antitoxin (TA) systems widely distributed among bacteria. Activation of the TA system is believed to result in the bacterial entrance to the dormant state, inducing persistence, and the formation of biofilms. In this study after biofilm formation and the induction of persister cells in biofilms, the relative expression level of type II TA system genes (relE1/relB1, relE2/relB2, hipA/hipB, vapB/vapC, phd/doc, mazF/mazE) in K. pneumoniae isolates were examined by Real Time PCR. Findings showed an increase in the expression levels of type II TA system genes in biofilm formers and an upregulation of all genes studied in biofilm persister cells except the phd/doc system. Our results can provide information about the potential role of the TA systems in biofilm production and also the biofilms persister cell formation. © 2020

Item Type: Article
Creators:
CreatorsEmail
Narimisa, N.UNSPECIFIED
Amraei, F.UNSPECIFIED
Kalani, B. S.UNSPECIFIED
Azarnezhad, A.UNSPECIFIED
Jazi, F. M.UNSPECIFIED
Keywords: Biofilm Klebsiella pneumoniae Persister cell Real-time PCR Type II TA system article controlled study nonhuman real time polymerase chain reaction toxin-antitoxin system upregulation
Divisions:
Journal or Publication Title: Gene Reports
Journal Index: Scopus
Volume: 21
Identification Number: https://doi.org/10.1016/j.genrep.2020.100846
ISSN: 24520144 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3129

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