Repository of Research and Investigative Information

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Ilam University of Medical Sciences

RAPD PCR and actA based molecular typing of L. monocytogenes isolated from human, food and domestic animals in northwest of Iran

Tue Mar 5 04:34:21 2024

(2020) RAPD PCR and actA based molecular typing of L. monocytogenes isolated from human, food and domestic animals in northwest of Iran. Meta Gene. ISSN 22145400 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Twenty-one Listeria monocytogenes isolates originated from food, animal, and clinical sources were evaluated by RAPD-PCR typing using four random primers (OPM-01, HLWL-74, HLWL-82, and HLWL85), both individually and in combination. The actA gene-typing and PCR-based serotyping were also used for the characterization of the isolates. Data were analyzed using the related software, and clusters were displayed in dendrogram. Among four primers, OPM-01 showed the highest discriminatory power. The dendrogram for human, food, and livestock isolates showed four different clusters (A-D) and two singletons. Using RAPD-PCR, lineages I, II, and the serogroups were discriminated. In actA gene-typing, 95 of isolates (all except one) belonged to actA type I. Results of PCR-serotyping and RAPD-PCR showed that animal isolates differed from food and clinical isolates. RAPD-PCR showed higher discriminatory power compared to other typing methods used in this study as isolates belonging to the same serotypes in PCR-based serotyping indicated different RAPD profiles. © 2020

Item Type: Article
Creators:
CreatorsEmail
Hemmati, A.UNSPECIFIED
Diba, K.UNSPECIFIED
Parvin, S.UNSPECIFIED
Ohadi, E.UNSPECIFIED
Sadeghi Kalani, B.UNSPECIFIED
Lotfollahi, L.UNSPECIFIED
Keywords: actA typing L. monocytogenes PCR-based serotyping RAPD typing article clinical assessment controlled study domestic animal human Iran Listeria monocytogenes livestock molecular typing nonhuman phylogenetic tree random amplified polymorphic DNA polymerase chain reaction serotype serotyping software
Divisions:
Journal or Publication Title: Meta Gene
Journal Index: Scopus
Volume: 26
Identification Number: https://doi.org/10.1016/j.mgene.2020.100790
ISSN: 22145400 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3128

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