Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Toxoplasma gondii ROP38 protein: Bioinformatics analysis for vaccine design improvement against toxoplasmosis

Sat Dec 10 03:32:03 2022

(2020) Toxoplasma gondii ROP38 protein: Bioinformatics analysis for vaccine design improvement against toxoplasmosis. Microbial Pathogenesis. p. 104488. ISSN 1096-1208 (Electronic) 0882-4010 (Linking)

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Official URL: https://www.ncbi.nlm.nih.gov/pubmed/32916240

Abstract

Rhoptry proteins (ROPs) play a significant role in various stages of Toxoplasma gondii (T. gondii) life cycle, being critical for both invasion and intracellular survival. ROP38 is a key manipulator of host gene expression and has a function in tachyzoite to bradyzoite conversion. In this study, we've employed various bioinformatics online tools for immunogenicity prediction of ROP38 protein, comprising physico-chemical, antigenic and allergenic profiles, transmembrane domain, subcellular localization, post-translational modification (PTM) sites, secondary and 3D structure, B-cell, MHC-binding and cytotoxic T-lymphocyte (CTL) epitopes. The findings showed 54 PTM sites without a transmembrane domain. Also, ROP38 was proved a non-allergenic and antigenic protein. The protein had Sec signal peptide (Sec/SPI) with 0.8762 likelihood. The secondary structure included 52.68 random coil, 29.57 alpha helix and 17.74 extended strand. Based on Ramachandran plot output for refined model, 95.3, 3.4, and 1.4 of amino acid residues were incorporated in the favored, allowed, and outlier regions, respectively. B-cell epitopes TFPGDDIQTSS (67-72) and KAKNKWGRTRYTLQG (207-221) as well as T-cell epitope LSPVGFFTAL (6-15) possessed the highest antigenic index in the protein sequence. This paper is a premise for further researches, and provides insights for the development of a suitable vaccine against toxoplasmosis. More empirical studies are required using the ROP38 alone or in combination with other antigens/epitopes in the future.

Item Type: Article
Creators:
CreatorsEmail
Nosrati, M. C.UNSPECIFIED
Ghasemi, E.UNSPECIFIED
Shams, M.UNSPECIFIED
Shamsinia, S.UNSPECIFIED
Yousefi, A.UNSPECIFIED
Nourmohammadi, H.UNSPECIFIED
Javanmardi, E.UNSPECIFIED
Kordi, B.UNSPECIFIED
Majidiani, H.UNSPECIFIED
Ghaffari, A. D.UNSPECIFIED
Shakarami, F.UNSPECIFIED
Keywords: Bioinformatics analysis Immunization Rop38 Toxoplasma gondii Vaccine
Divisions:
Page Range: p. 104488
Journal or Publication Title: Microbial Pathogenesis
Journal Index: Pubmed
Volume: 149
Identification Number: https://doi.org/10.1016/j.micpath.2020.104488
ISSN: 1096-1208 (Electronic) 0882-4010 (Linking)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3105

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