Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Chemical characterization and neuroprotective properties of copper nanoparticles green-synthesized bynigella sativaL. seed aqueous extract against methadone-induced cell death in adrenal phaeochromocytoma (PC12) cell line

Wed Sep 28 23:21:03 2022

(2020) Chemical characterization and neuroprotective properties of copper nanoparticles green-synthesized bynigella sativaL. seed aqueous extract against methadone-induced cell death in adrenal phaeochromocytoma (PC12) cell line. Journal of Experimental Nanoscience. pp. 280-296. ISSN 1745-8080

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Abstract

Recently, scientists have used the metallic nanoparticles especially copper nanoparticles for formulating many new neuroprotective supplements in the field of neurology. Also, we know the role ofNigella sativaL. in increasing the physiological activities of the central nervous system in traditional medicine. In the present study, we decided to prepare and formulate a new neuroprotective supplement (copper nanoparticles in aqueous medium usingN. sativaseed aqueous extract) in thein vitrocondition. The organometallic chemistry tests such as Fourier Transformed Infrared Spectroscopy (FT-IR), UV-Visible Spectroscopy (UV-Vis), Field Emission Scanning Electron Microscopy (FE-SEM), and Transmission Electron Microscopy (TEM) were used for characterizing of copper nanoparticles. In the FT-IR test, the presence of many antioxidant compounds with related bonds caused excellent condition for reducing copper in the copper nanoparticles. In UV-Vis, the clear peak in the wavelength of 569 nm showed the copper nanoparticles formation. Also, in the TEM and FE-SEM images, the copper nanoparticles had the size of 19.5 nm. In the biological part of the current study, methadone significantly (p <= 0.01) decreased cell viability, mitochondrial membrane potential, and increased inflammatory cytokines concentrations, caspase-3 activity, and DNA fragmentation. CuNPs-treated cell cutlers significantly (p <= 0.01) increased cell viability and mitochondrial membrane potential, and decreased inflammatory cytokines concentrations, caspase-3 activity, and DNA fragmentation in the high concentration of methadone-treated adrenal phaeochromocytoma (PC12) cells. For investigating the antioxidant properties of copper nanoparticles, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) test was used in the presence of butylated hydroxytoluene as the positive control. The copper nanoparticles inhibited half of the DPPH molecules in the concentration of 171 mu g/mL. In this study, we concluded that copper nanoparticles biosynthesized byN. sativaL. seed suppressed methadone-induced cell death in PC12 cells.

Item Type: Article
Creators:
CreatorsEmail
Yan, W.UNSPECIFIED
Liu, Y. T.UNSPECIFIED
Mansooridara, S.UNSPECIFIED
Kalantari, A. S.UNSPECIFIED
Sadeghian, N.UNSPECIFIED
Taslimi, P.UNSPECIFIED
Zangeneh, A.UNSPECIFIED
Zangeneh, M. M.UNSPECIFIED
Keywords: Chemical characterization copper nanoparticles neuroprotective supplement methadone PC12 aspartate nmda receptor maintenance patients anticancer activity cancer impairment apoptosis toxicity growth Chemistry Science & Technology - Other Topics Materials Science Physics
Divisions:
Page Range: pp. 280-296
Journal or Publication Title: Journal of Experimental Nanoscience
Journal Index: ISI
Volume: 15
Number: 1
Identification Number: https://doi.org/10.1080/17458080.2020.1778167
ISSN: 1745-8080
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3093

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