Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Suppressor capacity of copper nanoparticles biosynthesized using Crocus sativus L. leaf aqueous extract on methadone-induced cell death in adrenal phaeochromocytoma (PC12) cell line

Wed Sep 28 21:54:47 2022

(2020) Suppressor capacity of copper nanoparticles biosynthesized using Crocus sativus L. leaf aqueous extract on methadone-induced cell death in adrenal phaeochromocytoma (PC12) cell line. Scientific Reports. p. 15. ISSN 2045-2322

Full text not available from this repository.

Official URL: http://apps.webofknowledge.com/InboundService.do?F...

Abstract

In this research, we prepared and formulated a neuroprotective supplement (copper nanoparticles in aqueous medium utilizing Crocus sativus L. Leaf aqueous extract) for determining its potential against methadone-induced cell death in PC12. The results of chemical characterization tests i.e., FE-SEM, FT-IR, XRD, EDX, TEM, and UV-Vis spectroscopy revealed that the study showed that copper nanoparticles were synthesized in the perfect way possible. In the TEM and FE-SEM images, the copper nanoparticles were in the mean size of 27.5 nm with the spherical shape. In the biological part of the present research, the Rat inflammatory cytokine assay kit was used to measure the concentrations of inflammatory cytokines. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) test was used to show DNA fragmentation and apoptosis. Caspase-3 activity was assessed by the caspase activity colorimetric assay kit and mitochondrial membrane potential was studied by Rhodamine123 fluorescence dye. Also, the cell viability of PC12 was measured by trypan blue assay. Copper nanoparticles-treated cell cutlers significantly (p <= 0.01) decreased the inflammatory cytokines concentrations, caspase-3 activity, and DNA fragmentation and they raised the cell viability and mitochondrial membrane potential in the high concentration of methadone-treated PC12 cells. The best result of neuroprotective properties was seen in the high dose of copper nanoparticles i.e., 4 mu g. According to the above results, copper nanoparticles containing C. sativus leaf aqueous extract can be used in peripheral nervous system treatment as a neuroprotective promoter and central nervous system after approving in the clinical trial studies in humans.

Item Type: Article
Creators:
CreatorsEmail
Zhang, P.UNSPECIFIED
Cui, J.UNSPECIFIED
Mansooridara, S.UNSPECIFIED
Kalantari, A. S.UNSPECIFIED
Zangeneh, A.UNSPECIFIED
Zangeneh, M. M.UNSPECIFIED
Sadeghian, N.UNSPECIFIED
Taslimi, P.UNSPECIFIED
Bayat, R.UNSPECIFIED
Sen, F.UNSPECIFIED
Keywords: nanostructure voltammetric sensor aspartate nmda receptor carbon-paste electrode green synthesis ionic liquid maintenance patients anticancer activity multiple-sclerosis glycyrrhiza-glabra ascorbic-acid Science & Technology - Other Topics
Divisions:
Page Range: p. 15
Journal or Publication Title: Scientific Reports
Journal Index: ISI
Volume: 10
Number: 1
Identification Number: https://doi.org/10.1038/s41598-020-68142-8
ISSN: 2045-2322
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3056

Actions (login required)

View Item View Item