Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Haloperidol's effect on the expressions of TGFB, NT-3, and BDNF genes in cultured rat microglia

Mon May 20 15:31:05 2024

(2020) Haloperidol's effect on the expressions of TGFB, NT-3, and BDNF genes in cultured rat microglia. Basic and Clinical Neuroscience. pp. 49-58. ISSN 2008126X (ISSN)

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Introduction: Microglia, small glial cells, i.e. mesodermal in origin and found in the brain and spinal cord, play a key role in the maintenance of neurons and immune defense. Haloperidol, an antipsychotic drug, is used to treat numerous neurological and neurodegenerative disorders. Its mechanism is not understood; however, haloperidol may result in Wnt signaling pathway activation. This study aimed to activate the Wnt signaling pathway using haloperidol and determining the effect of GSK3 inhibition on the expression of TGFB, NT-3, and BDNF genes in cultured rat microglia. Methods: Microglia isolation was conducted, and the immunohistochemistry technique was performed to confirm microglia purity. The RNA extraction was followed by cDNA synthesis. Real-time RT-PCR was used to evaluate any significant changes in the expression level of these genes. Results: The three gene expressions in microglia were proportional to the different concentrations of the drug. More concentration of drugs resulted in higher levels of expression of these genes. Besides, the haloperidol did not affect the expression of the beta-actin gene as the reference gene. Conclusion: The obtained results supported the beneficial use of haloperidol in targeted microglia therapy. This study can be a breakthrough in neurology research. © 2020 Iran University of Medical Sciences. All rights reserved.

Item Type: Article
Forouzandeh, H.UNSPECIFIED
Khalafkhany, D.UNSPECIFIED
Foruozandeh, H.UNSPECIFIED
Keywords: BDNF Haloperidol Microglia NT-3 TGFB beta actin brain derived neurotrophic factor complementary DNA glycogen synthase kinase 3 neurotrophin 3 transforming growth factor beta Wnt protein animal cell animal experiment Article cell isolation controlled study DNA synthesis drug effect enzyme inhibition gene expression immunohistochemistry newborn nonhuman rat real time polymerase chain reaction real time reverse transcription polymerase chain reaction RNA extraction Wnt signaling
Page Range: pp. 49-58
Journal or Publication Title: Basic and Clinical Neuroscience
Journal Index: Scopus
Volume: 11
Number: 1
Identification Number:
ISSN: 2008126X (ISSN)
Depositing User: مهندس مهدی شریفی

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