Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Investigation of the effect of hydroalcoholic mumie extract on expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kappa-B ligand (RANKL)

Wed Aug 10 16:16:38 2022

(2020) Investigation of the effect of hydroalcoholic mumie extract on expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kappa-B ligand (RANKL). Scientific Journal of Kurdistan University of Medical Sciences. pp. 120-129. ISSN 1560652X (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Background and Aim: Osteoporosis is one of the most common bone diseases that is caused by an imbalance between the activity of osteoblasts and osteoclasts. Osteoprotegerin (OPG) plays an active role in inhibiting osteoclasts by binding to the receptor activator of nuclear factor kappa-B ligand (RANKL). The balance in the OPG/RANKL ratio is important in bone remodeling. The current study investigated and compared the effects of mumie extract and chemical drugs on cell proliferation, expression of OPG and RANKL in MG63 cells. Materials and Methods: The effects of 100, 200 and 300 μg/ml of mumie extract on cell proliferation, expression of OPG and RANKL in MG63 cells were investigated in experimental groups, positive and negative control groups. Data were analyzed by ANOVA test. Results: Significant increases were observed in the proliferation of MG63 cells after exposure to 100 and 200 μg/ml concentrations of mumie extract. 200 μg/ml concentration of the extract significantly decreased the expression of RANKL and increased the expression of OPG. It also increased OPG/RANKL ratio significantly in the experimental groups, compared to those in the negative control groups. Although the effects of 100 μg/ml and 200 μg/ml concentrations were similar to each other, they showed no significant effects on the variables. 300 μg/ml concentration of the extract showed a reverse and insignificant effect. Conclusion: 200 μg/ml concentration of mumie extract had a significant effect on the expression of two important factors in bone remodeling. Therefore, individuals susceptible to osteoporosis can use mumie extract as an alternative to chemical drugs. © 2018 the Author (s).

Item Type: Article
Creators:
CreatorsEmail
Azizpour, Y.UNSPECIFIED
Azizi, M.UNSPECIFIED
Asadollahi, K.UNSPECIFIED
Abbasi, N.UNSPECIFIED
Aidy, A.UNSPECIFIED
Karimi, E.UNSPECIFIED
Keywords: Mumie extract Osteoblasts Osteoporosis alcohol biological product osteoclast differentiation factor osteoprotegerin unclassified drug Article bone remodeling cell proliferation controlled study human human cell metabolic balance metabolic parameters MG-63 cell line osteoblast osteoclast osteoprotegerin osteoclast differentiation factor ratio protein expression
Divisions:
Page Range: pp. 120-129
Journal or Publication Title: Scientific Journal of Kurdistan University of Medical Sciences
Journal Index: Scopus
Volume: 24
Number: 6
ISSN: 1560652X (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/2863

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