Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Evaluation of cell-penetrating peptide–peptide nucleic acid effect in the inhibition of cagA in Helicobacter pylori

Wed Jul 24 10:20:40 2024

(2020) Evaluation of cell-penetrating peptide–peptide nucleic acid effect in the inhibition of cagA in Helicobacter pylori. Acta Microbiologica Et Immunologica Hungarica. pp. 66-72. ISSN 12178950 (ISSN)

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Helicobacter pylori is the most common cause of chronic infection in human and is associated with gastritis, peptic ulcer disease, and adenocarcinoma of mucosa-associated lymphoid tissue cells. Peptide nucleic acid (PNA) is a synthetic compound, which can inhibit the production of a particular gene. This study aimed to investigate the effect of PNA on inhibiting the expression of cagA. After confirmation of the desired gene by polymerase chain reaction (PCR), the antisense sequence was designed against cagA gene. The minimum inhibitory concentrations of conjugated PNA against H. pylori was determined. The effect of the compound on the expression level of the cagA was investigated in HT29 cell culture using real-time PCR. The results showed 2 and 3 log reduction in bacterial count after 8- and 24-h treatment with 4 and 8 μM of the compound, respectively. The lowest expression level of the cagA gene was observed at a concentration of 8 μM after 6 h. The results of this study showed that cell-penetrating peptide antisense can be employed as effective tools for inhibiting the target gene mRNA for various purposes. Moreover, further research is necessary to assess the potency, safety, and pharmacokinetics of CPP-PNAs for clinical prevention and treatment of infections due to H. pylori. © 2019 Akadémiai Kiadó, Budapest.

Item Type: Article
Mohammadzadeh, R.UNSPECIFIED
Keywords: Antisense Cell culture Helicobacter pylori Peptide nucleic acid Real-time PCR CagA protein cell penetrating peptide messenger RNA amino acid sequence antisense vector Article bacterial count bacterial virulence concentration (parameter) controlled study drug conjugation drug efficacy evaluation study gene targeting HT-29 cell line human human cell minimum inhibitory concentration nonhuman nonviral gene delivery system polymerase chain reaction protein expression level real time polymerase chain reaction
Page Range: pp. 66-72
Journal or Publication Title: Acta Microbiologica Et Immunologica Hungarica
Journal Index: Scopus
Volume: 67
Number: 1
Identification Number:
ISSN: 12178950 (ISSN)
Depositing User: مهندس مهدی شریفی

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