Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Persister cells formation and expression of type II Toxin-Antitoxin system genes in Brucella melitensis (16M) and Brucella abortus (B19)

Thu Aug 11 16:28:17 2022

(2020) Persister cells formation and expression of type II Toxin-Antitoxin system genes in Brucella melitensis (16M) and Brucella abortus (B19). Iranian journal of pathology. pp. 127-133. ISSN 1735-5303 (Print) 1735-5303 (Linking)

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Official URL: https://www.ncbi.nlm.nih.gov/pubmed/32215028

Abstract

Background & Objective: Persister cells are defined as a subpopulation of bacteria that are capable of reducing their metabolism and switching to dormancy in stress conditions. Persister cells formation has been attributed to numerous mechanisms, including stringent response and Toxin-Antitoxin (TA) systems. This study aimed to investigate the hypothetical role of TA systems in persister cells formation of Brucella strains by evaluating toxins of type II TA systems (RelE, Fic, Brn T, cogT) expression. Methods: Brucella strains treated with a lethal dose of gentamicin and ampicillin and to determine the number of surviving cells, bacterial colonies were counted at different time intervals. The role of TA systems in persister cell formation was then determined by toxin expression levels using qRT- PCR method. Results: Our results showed the viability of persister cells after 7 h. The results of relative qRT- PCR showed higher levels of toxin gene expression due to stress conditions, suggesting the possible role of TA systems in persister cells formation and antibiotics tolerance. Conclusion: The results of this study showed that considering the importance of persistence and the tolerance to antibiotics, further studies on persister cells formation and related genes such as the TA system genes in Brucella strains might help us to identify the precise mechanisms leading to persister cells formation.

Item Type: Article
Creators:
CreatorsEmail
Amraei, F.UNSPECIFIED
Narimisa, N.UNSPECIFIED
Sadeghi Kalani, B.UNSPECIFIED
Lohrasbi, V.UNSPECIFIED
Masjedian Jazi, F.UNSPECIFIED
Keywords: Brucella abortus Brucella melitensis Persister cell Real-time PCR TA systems
Divisions:
Page Range: pp. 127-133
Journal or Publication Title: Iranian journal of pathology
Journal Index: Pubmed
Volume: 15
Number: 2
Identification Number: https://doi.org/10.30699/ijp.2020.118902.2294
ISSN: 1735-5303 (Print) 1735-5303 (Linking)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/2810

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