Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

The effects of mumie extract on cell proliferation and enzyme expression of human osteoblast-like cells (MG63)

Wed Aug 10 15:02:21 2022

(2019) The effects of mumie extract on cell proliferation and enzyme expression of human osteoblast-like cells (MG63). Journal of stem cells & regenerative medicine. pp. 18-23. ISSN 0973-7154 (Print) 0973-7154 (Linking)

Full text not available from this repository.

Official URL: https://www.ncbi.nlm.nih.gov/pubmed/31983854

Abstract

Background: Mumie, as an inorganic and semi-solid herbal substance, could be obtained from crevice caves and is used for bone diseases in traditional medicine. This study investigated the effects of this substance on the expression of bone alkaline phosphatase (BALP) enzyme as well as proliferation and mortality rates of MG63 human osteoblast-like cells. Materials and methods: The MG63 cells were cultured and the effect of 100, 200 and 300 mug/ml of mumie extract on cell viability were compared with zoledronic acid and estradiol valerate as positive controls, as well as with MG63 cells alone as the negative control group. The activity rate of the BALP enzyme was also assessed. Results: During 48 hours of the study period, the concentrations of 100 and 200mug/ml of mumie extract increased the proliferation rate and decreased the mortality rate of MG63 cells significantly; however, the concentration of 300mug/ml decreased the proliferation rate and increased the mortality rate of the cells. Also, BALP enzyme expression was slightly affected by 100 and 200 mug/ml of mumie extract whilst it was significantly decreased by the concentration of 300 mug/ml. Conclusion: This study showed that mumie extract has an increasing effect on proliferation rate and a decreasing effect on the mortality rate of osteoblast cells in low concentrations; however, the higher concentrations of this substance could be toxic and effect inversely.

Item Type: Article
Creators:
CreatorsEmail
Abbasi, N.UNSPECIFIED
Azizpour, Y.UNSPECIFIED
Azizi, M.UNSPECIFIED
Karimi, E.UNSPECIFIED
Aidy, A.UNSPECIFIED
Asadollahi, K.UNSPECIFIED
Keywords: Mg63 Mumie extract alkaline phosphatase cell proliferation osteoblast
Divisions:
Page Range: pp. 18-23
Journal or Publication Title: Journal of stem cells & regenerative medicine
Journal Index: Pubmed
Volume: 15
Number: 2
ISSN: 0973-7154 (Print) 0973-7154 (Linking)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/2764

Actions (login required)

View Item View Item