Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Effects of transplanted olfactory ensheathing cells on functional improvement and axonal regeneration in acute and delayed spinal cord injury in rats: A comparative study

Thu Aug 18 18:38:17 2022

(2019) Effects of transplanted olfactory ensheathing cells on functional improvement and axonal regeneration in acute and delayed spinal cord injury in rats: A comparative study. Journal of the Anatomical Society of India. pp. 1-6. ISSN 0003-2778

Full text not available from this repository.

Official URL: http://apps.webofknowledge.com/InboundService.do?F...

Abstract

Introduction: Cell therapy is known as one of the most common methods used for treating a spinal cord injury (SCI); particularly, olfactory ensheathing cells (OECs), which have attracted much more attention among scholars due to their properties such as promotion of axonal regeneration, remyelination, and angiogenesis. Thus, the present study compared the effects of transplanted OECs on functional improvement and axonal regeneration of contused rats during acute and delayed phases. Material and Methods: For this purpose, a total of 56 male Wistar rats were randomly divided into eight groups including sham, control, three vehicle groups (immediately, 3 and 7 days after injury), and three cell transplantation groups (immediately, 3 and 7 days following injury). The sham group had experienced only laminectomy and other groups had undergone the SCI. The olfactory mucosa of the 7-day-old rat pups was also used for cell culture. The cell type was then confirmed by immunocytochemistry. In the vehicle and cell transplantation groups, the cell culture medium was injected by itself or accompanied by cells, respectively. Subsequently, the motor function was evaluated. Finally, luxol fast blue (LFB) staining was used for histological assessment. Results: Motor test results showed an increase in the Basso, Beattie, and Bresnahan locomotor scale scores immediately after injury in the transplantation group compared to those in other two cell-treated groups; but it was not significant. Discussion and Conclusion: Based on the LFB staining results, the regeneration rate in the transplantation group immediately after injury was considerably higher than that in two other treated groups. Therefore, considering these findings, it seemed that cell transplantation immediately after injury was better than that 3 and 7 days following injury.

Item Type: Article
Creators:
CreatorsEmail
Heydarizadi, S.UNSPECIFIED
Abbasi, N.UNSPECIFIED
Asadollahi, K.UNSPECIFIED
Rezaee, S.UNSPECIFIED
Moradipour, A.UNSPECIFIED
Azizi, M.UNSPECIFIED
Keywords: Cell transplantation contusion olfactory ensheathing cells spinal cord injury promote locomotor recovery lamina-propria schwann-cell transection bulb Anatomy & Morphology
Divisions:
Page Range: pp. 1-6
Journal or Publication Title: Journal of the Anatomical Society of India
Journal Index: ISI
Volume: 68
Number: 1
Identification Number: https://doi.org/10.4103/jasi.Jasi₂₁₁₉
ISSN: 0003-2778
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/2743

Actions (login required)

View Item View Item