Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Ethnomedicinal plant-extract-assisted green synthesis of iron nanoparticles using Allium saralicum extract, and their antioxidant, cytotoxicity, antibacterial, antifungal and cutaneous wound-healing activities

Wed May 22 12:54:57 2024

(2020) Ethnomedicinal plant-extract-assisted green synthesis of iron nanoparticles using Allium saralicum extract, and their antioxidant, cytotoxicity, antibacterial, antifungal and cutaneous wound-healing activities. Applied Organometallic Chemistry. p. 15. ISSN 0268-2605

Full text not available from this repository.

Official URL: http://apps.webofknowledge.com/InboundService.do?F...

Abstract

The aim of the experiment was the evaluation of antioxidant, cytotoxicity, antibacterial, antifungal and cutaneous wound-healing activities of green synthesized iron nanoparticles using Allium saralicum R.M. Fritsch leaves (FeNPs@AS). These nanoparticles were spherical with a size range of 40-45 nm, and were characterized using various analysis techniques including ultraviolet-visible spectroscopy to determine the presence of FeNPs@AS in the solution. We studied functional groups of A. saralicum extract in the reduction and capping process of FeNPs@AS by Fourier transform-infrared spectroscopy; crystallinity and FCC planes by X-ray diffraction pattern; and surface morphology, shapes and size of FeNPs@AS by scanning electron microscopy and transmission electron microscopy. Agar diffusion tests were done to determine the antibacterial and antifungal characteristics. FeNPs@AS prevented the growth of all bacteria and removed them at 2-8 mg/ml concentrations (P <= 0.01). In the case of antifungal potentials of FeNPs@AS, they inhibited the growth of all fungi and destroyed them at 2-4 mg/ml concentrations (P <= 0.01). The 2,2-diphenyl-1-picrylhydrazyl test revealed similar antioxidant potentials for FeNPs@AS and butylated hydroxytoluene. The synthesized FeNPs@AS had great cell viability dose-dependently and indicated this method was non-toxic. For the in vivo experiment, after creating the cutaneous wound, the rats were randomly divided into six groups: treatment with 0.2 FeNPs@AS ointment; treatment with 0.2 A. saralicum ointment; treatment with 0.2 FeCl3 center dot 6H(2)O ointment; treatment with 3 tetracycline ointment; treatment with Eucerin basal ointment; and untreated control. These groups were treated for 10 days. Use of FeNPs@AS ointment in the treatment groups significantly decreased (P <= 0.01) the wound area, total cells, neutrophils and lymphocytes, and significantly raised (P <= 0.01) the wound contracture, hydroxyl proline, hexosamine, hexuronic acid, fibrocyte and fibrocytes/fibroblast rate compared with other groups. These results show that the inclusion of A. saralicum extracts improves the therapeutical properties of FeNPs, which led to a significant enhancement in the antioxidant, non-cytotoxicity, antibacterial, antifungal and cutaneous wound-healing activities of the nanoparticles.

Item Type: Article
Creators:
CreatorsEmail
Zangeneh, A.UNSPECIFIED
Zangeneh, M. M.UNSPECIFIED
Moradi, R.UNSPECIFIED
Keywords: Allium saralicum R M Fritsch antimicrobial cutaneous wound-healing antioxidant cytotoxicity iron nanoparticles silver nanoparticles essential oil leaves nanomaterials acid Chemistry
Divisions:
Page Range: p. 15
Journal or Publication Title: Applied Organometallic Chemistry
Journal Index: ISI
Volume: 34
Number: 1
Identification Number: https://doi.org/10.1002/aoc.5247
ISSN: 0268-2605
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/2713

Actions (login required)

View Item View Item