Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Novel and green synthesis of a nanopolymer and its use as a drug delivery system of silibinin and silymarin extracts in the olfactory ensheathing cells of rats in normal and high-glucose conditions

Fri Sep 30 01:01:38 2022

(2019) Novel and green synthesis of a nanopolymer and its use as a drug delivery system of silibinin and silymarin extracts in the olfactory ensheathing cells of rats in normal and high-glucose conditions. Rsc Advances. pp. 38912-38927.

Full text not available from this repository.

Official URL: http://apps.webofknowledge.com/InboundService.do?F...

Abstract

Drug delivery systems have been of interest to researchers. The effects of synthesized nano-polymers as silibinin and silymarin extract drug delivery systems on olfactory ensheathing cells under normal and high-glucose conditions were studied. The structure of the nanopolymer was characterized by IR, HNMR, GPC, DLS, and AFM. The toxicity was evaluated by an MTT assay. The production of ROS and the generation of NO were evaluated by a probe of fluorescein diacetate and Griess methods, respectively. The expressions of the protein levels of ILK, VEGF, BDNF, and NGF were investigated by western blotting. The polymer size was between 50 and 150 nm. The loading capacities for silibinin and silymarin were 68.5 and 56.4, respectively, and the drug release for them was estimated at 54.1 and 50.8, respectively. In high-glucose conditions, the cells were protected (EC50 = 4.88 +/- 0.5 mu M) by silibinin and nanopolymer in low concentrations by reducing the amount of ROS and NO, maintaining ILK, reducing VEGF and increasing NGF and BDNF. Incubation with silibinin and nanopolymer at high concentrations increased cell death with LC50 = 57.36 +/- 2.5 and 43.18 +/- 1.8 mu M, respectively, in high-glucose states. Thus, the cells were protected by silibinin and nanopolymer in protective concentrations by reducing the amount of ROS and NO, maintaining ILK, reducing VEGF, and increasing BDNF and NGF. The mentioned mechanisms were totally reversed at high concentrations.

Item Type: Article
Creators:
CreatorsEmail
Shiri, S.UNSPECIFIED
Abbasi, N.UNSPECIFIED
Alizadeh, K.UNSPECIFIED
Karimi, E.UNSPECIFIED
Keywords: diabetic neuropathies polyester dendrimers silybum-marianum milk thistle nanoparticles cancer brain mellitus carriers peptide Chemistry
Divisions:
Page Range: pp. 38912-38927
Journal or Publication Title: Rsc Advances
Journal Index: ISI
Volume: 9
Number: 67
Identification Number: https://doi.org/10.1039/c9ra05608d
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/2692

Actions (login required)

View Item View Item