Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

A potential marker in brucellosis, long non coding RNA IFNG-AS1

Sun Sep 25 17:18:52 2022

(2019) A potential marker in brucellosis, long non coding RNA IFNG-AS1. Molecular Biology Reports. pp. 6495-6500. ISSN 0301-4851

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Abstract

Brucellosis is the most common bacterial zoonotic infection. This pathogen may survive and sustain in host. The aim of this study is to define relationship between long noncoding (lnc) RNA-IFNG-AS1 and interferon gamma (IFN-gamma) in different groups of patients with brucellosis compared to control group. In this study, associations of lncRNA IFNG-AS1 expression with secretion of IFN-gamma level in Sixty patients with brucellosis, which were divided into 3 groups (acute, chronic and relapse groups), as a case group were compared with 20 subjects with negative serological tests and brucellosis clinical manifestation as a control group. In this regard, RNA were extracted from isolated peripheral blood mononuclear cells (PBMCs). LncRNA IFNG-AS1, T-box transcription factor (T-bet) and IFN-gamma expressions were detected using quantitative polymerase chain reaction (qPCR). Serum level IFN-gamma was assessed using enzyme linked immunosorbent assay (ELISA). The results showed that expression level of LncRNA IFNG-AS1, T-bet and IFN-gamma increased significantly in all patient groups in compared to healthy subjects (P<0.0001, P<0.01, P<0.001). However, there was no significant difference in T-bet expression between chronic and healthy groups (P=0.98). Additionally, further analysis revealed that the serum level of IFN-gamma in acute and relapsed groups were higher than control group (P<0.0001, P<0.001). The effective role of IFNG-AS1 in many protective actions, including enhancing the expression of INF-gamma in the immune response of brucellosis patients, revealed new potential marker, LncRNA IFNG-AS1 in screening, diagnosis or treatment of brucellosis.

Item Type: Article
Creators:
CreatorsEmail
Gheitasi, R.UNSPECIFIED
Jourghasemi, S.UNSPECIFIED
Pakzad, I.UNSPECIFIED
Sarmadi, V. H.UNSPECIFIED
Samieipour, Y.UNSPECIFIED
Sekawi, Z.UNSPECIFIED
Jalilian, F. A.UNSPECIFIED
Keywords: Brucellosis Cytokine Interferon-gamma T-bet lncRNA IFNG-AS1 noncoding rna tmevpg1 expression gene Biochemistry & Molecular Biology
Divisions:
Page Range: pp. 6495-6500
Journal or Publication Title: Molecular Biology Reports
Journal Index: ISI
Volume: 46
Number: 6
Identification Number: https://doi.org/10.1007/s11033-019-05095-w
ISSN: 0301-4851
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/2691

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