Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Rapid and direct molecular detection of Streptococcus pneumoniae and Haemophilus influenzae isolated in oropharynx and nasal cavity of children

Wed Sep 28 12:07:02 2022

(2020) Rapid and direct molecular detection of Streptococcus pneumoniae and Haemophilus influenzae isolated in oropharynx and nasal cavity of children. New Microbes and New Infections. p. 5.

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Abstract

There is a direct correlation between asymptomatic oropharyngeal colonization by pathogenic bacteria and the prevalence of paediatric respiratory infections. Streptococcus pneumoniae and Haemophilus influenzae are common cause of serious bacterial infections such as meningitis in children. This study aimed to monitor healthy children to detect asymptomatic carriers of S. pneumoniae and H. influenzae. In the present cross-sectional study, real-time PCR assay was developed and evaluated in comparison with culture for direct detection of S. pneumoniae and H. influenzae in 123 oropharynx and nasal cavity specimens from healthy children in Ilam, Iran. In addition, virulence factor (ply and hpd) and iron uptake (tbpA and piuA) genes were evaluated by PCR. Our results demonstrated that among all isolates only 14 S. pneumoniae and eight H. influenzae were identified by phenotypic methods, whereas 37 and 21 S. pneumoniae and H. influenzae were identified, respectively, by real-time PCR assay. All S. pneumoniae and H. influenzae isolates possessed tbpA and piuA genes. Also, 81 (30/37) of S. pneumoniae isolates were positive for ply gene, which encoded pneumolysin, as well as 90 (19/21) of H. influenzae isolates were positive for hpd gene. Simultaneous colonization of S. pneumoniae and H. influenzae could indicate the importance of monitoring of healthy children to identification of carriers. (C) 2019 Published by Elsevier Ltd.

Item Type: Article
Creators:
CreatorsEmail
Maleki, A.UNSPECIFIED
Mansournia, F.UNSPECIFIED
Ghafourian, S.UNSPECIFIED
Taherikalani, M.UNSPECIFIED
Pakzad, I.UNSPECIFIED
Mohammadi, J.UNSPECIFIED
Kouhsari, E.UNSPECIFIED
Mahmoudi, M.UNSPECIFIED
Sadeghifard, N.UNSPECIFIED
Keywords: Detection Haemophilus influenzae iron uptake real-time PCR assay Streptococcus pneumoniae virulence factors microbiota carriage Infectious Diseases Microbiology
Divisions:
Page Range: p. 5
Journal or Publication Title: New Microbes and New Infections
Journal Index: ISI
Volume: 33
Identification Number: https://doi.org/10.1016/j.nmni.2019.100632
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/2668

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