Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Inhibitory effects of Lactobacilli cocktail on HT-29 colon carcinoma cells growth and modulation of the Notch and Wnt/beta-catenin signaling pathways

Thu Aug 11 17:12:23 2022

(2020) Inhibitory effects of Lactobacilli cocktail on HT-29 colon carcinoma cells growth and modulation of the Notch and Wnt/beta-catenin signaling pathways. Microbial Pathogenesis. p. 9. ISSN 0882-4010

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Abstract

Probiotics could be considered as attractive candidates for preventing tumor growth through maintaining homeostasis. The aim of this study was to evaluate the inhibitory effect of a cocktail of five Lactobacillus species on human colorectal carcinoma cell line HT-29. The anti-proliferative and apoptotic effects of Lactobacilli cocktail were evaluated using MTT and flow cytometry tests, respectively. Quantitative real-time polymerase chain reaction (qPCR) was used to analyze the expression of several genes in the Notch (notch, hes1, msi1, and numb) and Wnt/beta-catenin (CTNNB1 and CCND1) pathways, following the treatment of HT-29 cells with Lactobacilli cocktail. The treatment by Lactobacilli cocktail induced a significant anti-proliferative effect and late stage apoptosis among the cancer cells (p < 0.05). Compared to the untreated cells, Lactobacilli cocktail induced the down-regulation of notch, hest, and msi1 genes and up-regulation of numb gene in the Notch pathway as well as the down-regulation of CTNNB1 and CCND1 genes in the Wnt/beta-catenin pathway in a time-dependent manner (p < 0.05). Conclusion: Lactobacilli cocktail was shown to have beneficial anti-tumor effects on HT-29 cells by modulating the Notch and Wnt/beta-catenin pathways; therefore, the use of Lactobacilli probiotics as nutritional supplements may prevent the incidence of colon cancer.

Item Type: Article
Creators:
CreatorsEmail
Ghanavati, R.UNSPECIFIED
Asadollahi, P.UNSPECIFIED
Shapourabadi, M. B.UNSPECIFIED
Razavi, S.UNSPECIFIED
Talebi, M.UNSPECIFIED
Rohani, M.UNSPECIFIED
Keywords: Lactobacilli Anti-proliferation Apoptosis Notch Wnt/beta-catenin nf-kappa-b probiotics apoptosis microbiota bacteria target roles wnt Immunology Microbiology
Divisions:
Page Range: p. 9
Journal or Publication Title: Microbial Pathogenesis
Journal Index: ISI
Volume: 139
Identification Number: https://doi.org/10.1016/j.micpath.2019.103829
ISSN: 0882-4010
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/2637

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