Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Evaluation of cell-penetrating peptide-peptide nucleic acid effect in the inhibition of cagA in Helicobacter pylori

Tue Nov 29 02:19:06 2022

(2020) Evaluation of cell-penetrating peptide-peptide nucleic acid effect in the inhibition of cagA in Helicobacter pylori. Acta Microbiologica Et Immunologica Hungarica. pp. 66-72. ISSN 1217-8950

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Abstract

Helicobacter pylori is the most common cause of chronic infection in human and is associated with gastritis, peptic ulcer disease, and adenocarcinoma of mucosa-associated lymphoid tissue cells. Peptide nucleic acid (PNA) is a synthetic compound, which can inhibit the production of a particular gene. This study aimed to investigate the effect of PNA on inhibiting the expression of cagA. After confirmation of the desired gene by polymerase chain reaction (PCR), the antisense sequence was designed against cagA gene. The minimum inhibitory concentrations of conjugated PNA against H. pylori was determined. The effect of the compound on the expression level of the cagA was investigated in HT29 cell culture using real-time PCR. The results showed 2 and 3 log reduction in bacterial count after 8- and 24-h treatment with 4 and 8 mu M of the compound, respectively. The lowest expression level of the cagA gene was observed at a concentration of 8 mu M after 6 h. The results of this study showed that cell-penetrating peptide antisense can be employed as effective tools for inhibiting the target gene mRNA for various purposes. Moreover, further research is necessary to assess the potency, safety, and pharmacokinetics of CPP-PNAs for clinical prevention and treatment of infections due to H. pylori.

Item Type: Article
Creators:
CreatorsEmail
Javanmard, Z.UNSPECIFIED
Kalani, B. S.UNSPECIFIED
Razavi, S.UNSPECIFIED
Farahani, N. N.UNSPECIFIED
Mohammadzadeh, R.UNSPECIFIED
Javanmard, F.UNSPECIFIED
Irajian, G.UNSPECIFIED
Keywords: Helicobacter pylori peptide nucleic acid antisense real-time PCR cell culture in-situ hybridization gastric-cancer antimicrobial resistance rapid identification virulence factors bacterial caga infection risk culture vaca Immunology Microbiology
Divisions:
Page Range: pp. 66-72
Journal or Publication Title: Acta Microbiologica Et Immunologica Hungarica
Journal Index: ISI
Volume: 67
Number: 1
Identification Number: https://doi.org/10.1556/030.66.2019.032
ISSN: 1217-8950
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/2613

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