Repository of Research and Investigative Information

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Ilam University of Medical Sciences

Ocular Fungi: Molecular Identification and Antifungal Susceptibility Pattern to Azoles

Wed Nov 30 17:25:01 2022

(2020) Ocular Fungi: Molecular Identification and Antifungal Susceptibility Pattern to Azoles. Jundishapur Journal of Microbiology. p. 6. ISSN 2008-3645

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Abstract

Background: Treatment of ocular infection by fungi has become a problematic issue, particularly in deep lesion cases, because of the limited available antifungals and emerging resistance species. Objectives: The present study was designed for molecular identification and studying the antifungal susceptibility pattern of ocular fungi. Methods: Fifty-three ocular fungal isolates, including Fusarium spp., Aspergillus spp., yeast spp., and dematiaceous fungi were collected. Initial identification of each sample was performed using routine mycological techniques. ITS1-5.8SrDNA-ITS2 and translation elongation factor (TEF)-1 alpha regions were used for the identification and differentiation of ocular non-Fusarium and Fusarium fungal species, respectively. The antifungal susceptibility of itraconazole, voriconazole, and posaconazole was determined according to the CLSI guidelines (CLSI M38 and M60, 3rd ed.) for filamentous and yeast species, respectively. Results: Voriconazole and posaconazole showed excellent activity in all tested isolates; however, some of Fusarium, Aspergillus, and Curvularia strains showed minimum inhibitory concentration (MIC) >= 2 mu g/mL. The itraconazole showed different results in all species, and high MICs (>= 16 mu g/mL) were found. Conclusions: Finally, in the present study, we tried to identify species involved in fungal ocular infection using the molecular methods, which highlighted the importance of precise identification of species to choose an appropriate antifungal regime. On the other hand, our findings showed that antifungal susceptibility test is effective to reliably predict the in vivo response to therapy in infections; however, in fungal ocular infection cases, the penetration of antifungals may contribute to predict the outcome.

Item Type: Article
Creators:
CreatorsEmail
Soleimani, M.UNSPECIFIED
Salehi, Z.UNSPECIFIED
Fattahi, A.UNSPECIFIED
Lotfali, E.UNSPECIFIED
Yassin, Z.UNSPECIFIED
Ghasemi, R.UNSPECIFIED
Abedinifar, Z.UNSPECIFIED
Kouhsari, E.UNSPECIFIED
Ahmadkhani, F.UNSPECIFIED
Mirkalantari, S.UNSPECIFIED
Keywords: Fungal Ocular Infection Identification Fungus Drug Sensitivity Tests fusarium keratitis south-india voriconazole infections resistance natamycin Microbiology
Divisions:
Page Range: p. 6
Journal or Publication Title: Jundishapur Journal of Microbiology
Journal Index: ISI
Volume: 13
Number: 3
Identification Number: https://doi.org/10.5812/jjm.99922
ISSN: 2008-3645
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/2610

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