Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

PuraMatrix hydrogel enhances the expression of motor neuron progenitor marker and improves adhesion and proliferation of motor neuron-like cells

Thu Apr 18 23:57:08 2024

(2020) PuraMatrix hydrogel enhances the expression of motor neuron progenitor marker and improves adhesion and proliferation of motor neuron-like cells. Iranian Journal of Basic Medical Sciences. pp. 431-438. ISSN 2008-3866

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Objective(s): Cell therapy has provided clinical applications to the treatment of motor neuron diseases. The current obstacle in stem cell therapy is to direct differentiation of stem cells into neurons in the neurodegenerative disorders. Biomaterial scaffolds can improve cell differentiation and are widely used in translational medicine and tissue engineering. The aim of this study was to compare the efficiency of two-dimensional with a three-dimensional culture system in their ability to generate functional motor neuron-like cells from adipose-derived stem cells. Materials and Methods: We compared motor neuron-like cells derived from rat adipose tissue in differentiation, adhesion, proliferation, and functional properties on two-dimensional with three-dimensional culture systems. Neural differentiation was analyzed by immunocytochemistry for immature (Islet1) and mature (HB9, ChAT, and synaptophysin) motor neuron markers. Results: Our results indicated that the three-dimensional environment exhibited an increase in the number of Islet1. In contrast, two-dimensional culture system resulted in more homeobox gene (HB9), Choline Acetyltransferase (ChAT), and synaptophysin positive cells. The results of this investigation showed that proliferation and adhesion of motor neuron-like cells significantly increased in three-dimensional compared with two-dimensional environments. Conclusion: The findings of this study suggested that three-dimension may create a proliferative niche for motor neuron-like cells. Overall, this study strengthens the idea that three-dimensional culture may mimic neural stem cell environment for neural tissue regeneration.

Item Type: Article
Hamidabadi, H. G.UNSPECIFIED
Mirnajafi-Zadeh, J.UNSPECIFIED
Keywords: Motor neuron-like cells Nanoscaffolds Proliferation Stem cell therapy Three-dimension culture Tissue engineering embryonic stem-cells extracellular-matrix differentiation adipose transdifferentiation therapy Research & Experimental Medicine Pharmacology & Pharmacy
Page Range: pp. 431-438
Journal or Publication Title: Iranian Journal of Basic Medical Sciences
Journal Index: ISI
Volume: 23
Number: 4
Identification Number:
ISSN: 2008-3866
Depositing User: مهندس مهدی شریفی

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