Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

High expression of TIM-3 and KIM-1 in blood and urine of renal allograft rejection patients

Sat Oct 1 11:13:39 2022

(2017) High expression of TIM-3 and KIM-1 in blood and urine of renal allograft rejection patients. Transplant Immunology. pp. 11-20. ISSN 0966-3274

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Abstract

Background-T cell immunoglobulin and mucin domain 3 (TIM-3) is involved in alloimmune and autoimmune responses, as well as tolerance induction in kidney transplantation. Kidney injury molecule-1 (KIM-1) is highly expressed in epithelial cells of the injured proximal tubule. In this study, we have investigated both urinary and blood TIM-3 mRNA expressions, urinary KIM-1 mRNA expression, and urinary and serum KIM-1 proteins in renal allograft recipients diagnosed with acute allograft rejection (AR) and chronic allograft dysfunction (CAD), as well as those with well-functioning transplants (WFG). Methods: We divided 85 patients into the following groups: AR (n = 24), CAD (n = 19), and WFG (n = 42). TIM-3 and KIM-1 mRNA expressions were quantified using real-time reverse-transcription TaqMan probe polymerase chain reaction (RT-PCR). An ELISA test was used to measure the amount of KIM-1 protein in serum and urine samples. Results: AR and CAD patients had significantly greater urinary and blood TIM-3 mRNA expressions, urinary KIM-1 mRNA expression, and urinary and serum KIM-1 proteins compared to WFG patients. Receiver operating characteristic (ROC) analysis showed that these molecules discriminated Allograft rejections from WFG. Conclusion: Quantification of TIM-3 and KIM-1 mRNA expressions, along with KIM-1 protein measurements in urine and blood could be employed as promising tools for noninvasive diagnosis of allograft dysfunction.

Item Type: Article
Creators:
CreatorsEmail
Shahbaz, S. K.UNSPECIFIED
Pourrezagholi, F.UNSPECIFIED
Barabadi, M.UNSPECIFIED
Foroughi, F.UNSPECIFIED
Hosseinzadeh, M.UNSPECIFIED
Ahmadpoor, P.UNSPECIFIED
Nafar, M.UNSPECIFIED
Yekaninejad, M. S.UNSPECIFIED
Amirzargar, A.UNSPECIFIED
Keywords: TIM-3 KIM-1 Renal transplantation Acute allograft rejection Chronic allograft dysfunction kidney injury molecule-1 hepatitis-a virus transplant recipients alloimmune responses graft-survival messenger-rna biomarker cells dysfunction activation Immunology Transplantation
Divisions:
Page Range: pp. 11-20
Journal or Publication Title: Transplant Immunology
Journal Index: ISI
Volume: 43-44
Identification Number: https://doi.org/10.1016/j.trim.2017.07.002
ISSN: 0966-3274
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/258

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