Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Lactobacillus species inhibitory effect on colorectal cancer progression through modulating the Wnt/beta-catenin signaling pathway

Sat Apr 20 13:58:05 2024

(2020) Lactobacillus species inhibitory effect on colorectal cancer progression through modulating the Wnt/beta-catenin signaling pathway. Molecular and Cellular Biochemistry. pp. 1-13. ISSN 0300-8177

Full text not available from this repository.

Official URL:


Probiotic bacteria are known to exert a wide range of anticancer activities on their animal hosts. In the present study, the anticancer effect of a cocktail of several potential probiotic Lactobacillus species (potential probiotic L.C) was investigated in vitro and in vivo. MTT and Flow cytometry tests results showed that administration of live potential probiotic L.C significantly decreased the HT-29 and CT-26 cells proliferation and induced late apoptotis in a time-dependent manner. In addition, quantitative real-time polymerase chain reaction (qPCR) results showed that exposure of potential probiotic L.C to both HT-29 and CT-26 cells during the incubation times resulted in the upregulation (apc and CSNK1 epsilon for HT-29, CSNK1 epsilon and gsk3 beta for CT-26) and downregulation (CTNNB1, CCND1, pygo2, axin2 and id2) of the Wnt/beta- catenin pathway-related genes in a time-dependent manner. The significance of in vitro anticancer effect of potential probiotic L.C was further confirmed in an experimental tumor model. Data from the murine model of colorectal cancer (CRC) induced by Azoxymethane (AOM) and Dextran Sulfate Sodium (DSS) showed significantly alleviated inflammation and tumor development in AOM/DSS/L.C-injected mice compared to the AOM/DSS-injected mice. Tumor growth inhibition was accompanied by potential probiotic L.C-driven upregulation and downregulation of the Wnt/beta-catenin pathway-related genes, similar to the in vitro results. These results showed that potential probiotic L.C inhibited the tumor growth, and that its anticancer activity was at least partially mediated through suppressing the Wnt/beta-catenin pathway. Overall, the present study suggested that this probiotic could be used clinically as a supplement for CRC prevention and treatment. Graphic abstract

Item Type: Article
Keywords: Lactobacilli cocktail Anti proliferation Apoptosis Wnt beta-catenin pathway Azoxymethane induced colon-cancer gut microbiota probiotics transcription mechanisms plantarum complex target Cell Biology
Page Range: pp. 1-13
Journal or Publication Title: Molecular and Cellular Biochemistry
Journal Index: ISI
Volume: 470
Number: 1-2
Identification Number:
ISSN: 0300-8177
Depositing User: مهندس مهدی شریفی

Actions (login required)

View Item View Item