Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Combination of Antibiotics-Nisin Reduces the Formation of Persister Cell in Listeria monocytogenes

Sun Mar 26 19:51:22 2023

(2020) Combination of Antibiotics-Nisin Reduces the Formation of Persister Cell in Listeria monocytogenes. Microbial Drug Resistance. p. 8. ISSN 1076-6294

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Abstract

Persister cells are a subpopulation of bacteria with the ability of survival when exposed to lethal doses of antibiotics, and are responsible for antibiotic therapy failure and infection recurrences. In this study, we investigated persister cell formation and the role of nisin in combination with antibiotics in reducing persistence in Listeria monocytogenes. We also examined the expression of toxin-antitoxin (TA) systems in persister cells of L. monocytogenes to gain a better understanding of the effect of TA systems on persister cell formation. To induce persistence, L. monocytogenes were exposed to high doses of different antibiotics over a period of 24 hr, and the expression levels of TA system was genes were measured 5 hr after the addition of antibiotics by the quantitative reverse transcription-polymerase chain reaction (qRT-PCR) method. To investigate the effect of nisin, L. monocytogenes was exposed to a combination of nisin and antibiotics. According to our results, L. monocytogenes was highly capable of persister cell formation, and the combination of nisin and antibiotics resulted in reduced persistence. qRT-PCR results showed a significant increase in GNAT/RHH expression among the studied systems. Overall, our results demonstrated the potential of the combination of nisin and antibiotics in reducing persister cell formation, and emphasized the role of the GNAT/RHH system in bacterial persistence.

Item Type: Article
Creators:
CreatorsEmail
Narimisa, N.UNSPECIFIED
Kalani, B. S.UNSPECIFIED
Mohammadzadeh, R.UNSPECIFIED
Jazi, F. M.UNSPECIFIED
Keywords: Listeria monocytogenes nisin persister cells TA system real-time PCR toxin-antitoxin systems bacterial infections tolerance evolution survival Infectious Diseases Microbiology Pharmacology & Pharmacy
Divisions:
Page Range: p. 8
Journal or Publication Title: Microbial Drug Resistance
Journal Index: ISI
Identification Number: https://doi.org/10.1089/mdr.2020.0019
ISSN: 1076-6294
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/2568

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