Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Voriconazole resistance genes in Aspergillus flavus clinical isolates

Sat Dec 10 02:21:22 2022

(2020) Voriconazole resistance genes in Aspergillus flavus clinical isolates. Journal De Mycologie Medicale. p. 5. ISSN 1156-5233

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Abstract

Objective. - The present study was designed to discover novel biomarkers involved in voriconazole resistance in clinical isolates of Aspergillus flavus. Materials and methods. - Two voriconazole non-wild-type and two voriconazole-wild-type A. flavus clinical isolates were selected to evaluate possible molecular mechanism involved in A.flavus resistance to voriconazole using the mutation assessment, Quantitative real- time PCR of cyp51A and cyp51C genes and complementary DNA- amplified fragment length polymorphism technique. Results. - No mutations were seen in the cyp51A and cyp51C genes in voriconazole non-wild-type isolates compared to wild- type and reference strains. Regarding to mRNA expression results, no changes were observed in expression fold of cyp51A and cyp51C mRNA expression level in first non- wild- type isolate compared to wild-type isolate. For second isolate cyp51C mRNA expression level was down regulated (5.6 fold). The set of genes including ABC fatty acid transporter XM- 002375835 and aldehydereductase XM- 002376518 and three unknown functional genes were identified. Based on results, the over-expression of AKR1 and ABC fatty acid transporter in the voriconazole non- wild- type isolates suggests these genes could represent a novel molecular marker linked to the voriconazole resistance in A. flavus. Conclusion. - The results obtained in this study showed a novel finding as the authors identified AKR1 and ABC fatty acid transporter genes as possible voriconazole target genes in Iranian clinical isolates of A. flavus. (C) 2020 Published by Elsevier Masson SAS.

Item Type: Article
Creators:
CreatorsEmail
Zaini, F.UNSPECIFIED
Lotfali, E.UNSPECIFIED
Fattahi, A.UNSPECIFIED
Siddig, E.UNSPECIFIED
Farahyar, S.UNSPECIFIED
Kouhsari, E.UNSPECIFIED
Saffari, M.UNSPECIFIED
Keywords: Voriconazole Resistance gene Molecular markers Aspergillus flavus aldo-keto reductase azole resistance antimony resistance markers identification superfamily expression efflux yeast Mycology
Divisions:
Page Range: p. 5
Journal or Publication Title: Journal De Mycologie Medicale
Journal Index: ISI
Volume: 30
Number: 2
Identification Number: https://doi.org/10.1016/j.mycmed.2020.100953
ISSN: 1156-5233
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/2565

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