Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

The expression of type II TA system genes following exposure to the sub-inhibitory concentration of gentamicin and acid stress in Brucella spp

Thu Aug 11 17:11:47 2022

(2020) The expression of type II TA system genes following exposure to the sub-inhibitory concentration of gentamicin and acid stress in Brucella spp. Microbial Pathogenesis. p. 6. ISSN 0882-4010

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Abstract

Background: Brucellosis is one of the most common diseases that afflicts both humans and animals. Bacteria react to stress conditions using different mechanisms one of which is Toxin-Antitoxin (TA) systems. It is believed that the Toxin-Antitoxin (TA) systems have a key role in the chronicity of the disease. This study investigated the expression of TA system genes under acid and antibiotic stresses in Brucella spp. Methods: Fifty Brucella isolates (17 isolated from animals and 31 isolated from human specimens, and two standard strains) were analyzed using PCR (using two pairs of primers). Then, to determine the effects of subMIC of gentamicin on bacterial survival and growth, colony forming unit was quantitated and turbidity was assessed following the treatment of Brucella spp, with 1/2 MIC of gentamicin at different time intervals. Furthermore, the colony forming unit of Brucella spp, was assessed under acid stress (pH = 5.5) compared to the control (pH = 7.6). Moreover, the expression of TA system genes in Brucella spp, was evaluated 1 h after treatment using qRT-PCR method. Results: A total of 50 isolates, including 41 (82) Brucella melitensis and 7 (14) Brucella abortus with two standard strains Brucella melitensis (16 M) and Brucella abortus (B19) were investigated. Our results revealed the reduced growth of Brucella spp. in the presence of sub-MIC of gentamicin compared to the control. Furthermore, according to the results of qRT-PCR assay, gentamicin could increase the expression of TA system genes. Also, results of qRT-PCR showed that under acid stress, the expression of TA system gene COGT/COGAT decreased compared to the control. Conclusion: Although the exact role of the TA systems in response to stress is still unclear, our study provided information on the effect of the type II TA systems under the acid and antibiotic stress conditions. However, further studies are still required.

Item Type: Article
Creators:
CreatorsEmail
Amraei, F.UNSPECIFIED
Narimisa, N.UNSPECIFIED
Kalani, B. S.UNSPECIFIED
Mohammadzadeh, R.UNSPECIFIED
Lohrasbi, V.UNSPECIFIED
Jazi, F. M.UNSPECIFIED
Keywords: Brucella melitensis Brucella abortus Real-time polymerase chain reaction Toxin-antitoxin systems Acid stress Sub-inhibitory antibiotic concentrations toxin-antitoxin systems multiplex pcr assay persister cells resistance virulence protein ladder hipa Immunology Microbiology
Divisions:
Page Range: p. 6
Journal or Publication Title: Microbial Pathogenesis
Journal Index: ISI
Volume: 144
Identification Number: https://doi.org/10.1016/j.micpath.2020.104194
ISSN: 0882-4010
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/2555

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