Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

The effect of Descurainia sophia oil on methamphetamine-induced cell cytotoxicity and cell death in PC12

Thu Sep 29 08:25:51 2022

(2019) The effect of Descurainia sophia oil on methamphetamine-induced cell cytotoxicity and cell death in PC12. Comparative Clinical Pathology. pp. 1143-1152. ISSN 16185641 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Methamphetamine causes cytotoxicity and apoptosis in different cell lines. It seems that Descurainia sophia oil, as an East Asian folk herbal drug, can suppress the methamphetamine-induced cell death. In this study, protective effects of Descurainia sophia oil were followed up in methamphetamine-induced cell cytotoxicity in a neuron-like PC12 cell line. The viability, proliferation, and cytotoxicity of the cells were assessed by Trypan blue, MTT test, and lactate dehydrogenase (LDH) assay, respectively. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) test was performed to detect DNA fragmentation and apoptosis. Nitric oxide (NO) level was detected by Griese method. Interleukin-1β (IL-1β), IL-6, interferon gamma (INF-γ), and tumor necrosis factor alpha (TNF-α) pro-inflammatory cytokines were measured by Rat Kit V-Plex, and finally, caspase-3 activity was determined by spectrophotometry. Results showed that Descurainia sophia oil has cell death–suppressing effects on PC12 cells. It enhanced the cell viability and proliferation and also increased the cell cytotoxicity and cell death index in methamphetamine-treated PC12 cells. Also, it suppressed NO production, inflammatory cytokine production by flow cytometry, mitochondrial membrane depolarization, and caspase-3 activity in a dose-dependent manner. We concluded that Descurainia sophia oil suppresses the methamphetamine-induced cell death in PC12 cell due to reduction of NO production, inflammation, and inhibition of apoptosis cascade. © 2019, Springer-Verlag London Ltd., part of Springer Nature.

Item Type: Article
Creators:
CreatorsEmail
Sahraei, M.UNSPECIFIED
Zangeneh, M. M.UNSPECIFIED
Khanahmadi, M.UNSPECIFIED
Moradi, R.UNSPECIFIED
Kazemi, N.UNSPECIFIED
Zhaleh, H.UNSPECIFIED
Amini, K.UNSPECIFIED
Keywords: Cell cytotoxicity Cell death Descurainia sophia Methamphetamine Oil PC12
Divisions:
Page Range: pp. 1143-1152
Journal or Publication Title: Comparative Clinical Pathology
Journal Index: Scopus
Volume: 28
Number: 4
Identification Number: https://doi.org/10.1007/s00580-019-02968-1
ISSN: 16185641 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/2489

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