Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Dynamic variation of kidney injury molecule-1 mRNA and protein expression in blood and urine of renal transplant recipients: a cohort study

Sat Oct 1 12:44:08 2022

(2019) Dynamic variation of kidney injury molecule-1 mRNA and protein expression in blood and urine of renal transplant recipients: a cohort study. Clinical and experimental nephrology. pp. 1235-1249. ISSN 1437-7799 (Electronic) 1342-1751 (Linking)

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Official URL: https://www.ncbi.nlm.nih.gov/pubmed/31302846

Abstract

BACKGROUND: Acute renal dysfunction still constitutes a highly significant obstacle to renal transplantation outcome. Kidney injury molecule-1 is highly upregulated in proximal tubular cells and shed into the urine and blood circulation following kidney injury. The aim of current cohort study was to evaluate the urine KIM-1 (uKIM-1) mRNA expression level and its protein concentration in blood and urine samples to determine whether sequential monitoring of KIM-1 in renal allograft recipients is a reliable biomarker for predicting the clinical status and outcome. METHODS: Both uKIM-1 mRNA expression level and the level of serum and uKIM-1 protein concentration in the 52 renal transplant recipients were respectively quantified using real-time PCR and ELISA methods at 2, 90 and 180 days after transplantation. RESULT: KIM-1 mRNA and protein expression level in the blood and urine samples of patients with graft dysfunction was significantly higher than patients with well-functioning graft on days 2, 90 and 180 after transplantation. Receiver-operating characteristic curve analysis of mRNA and protein expression levels showed that urinary and blood KIM-1 at months 3 and 6 could predict acute renal dysfunction at 6 months and 1 year after transplantation. CONCLUSION: Sequential monitoring of uKIM-1 mRNA expression level and its protein concentration in the serum and urine samples of renal transplant patients suggests that KIM-1 could be a sensitive and specific biomarker for early diagnosis and prognosis of kidney allograft injury.

Item Type: Article
Creators:
CreatorsEmail
Keshavarz Shahbaz, S.UNSPECIFIED
Pourrezagholi, F.UNSPECIFIED
Nafar, M.UNSPECIFIED
Ahmadpoor, P.UNSPECIFIED
Barabadi, M.UNSPECIFIED
Foroughi, F.UNSPECIFIED
Hosseinzadeh, M.UNSPECIFIED
Yekaninejad, M. S.UNSPECIFIED
Amirzargar, A.UNSPECIFIED
Keywords: Graft dysfunction Kim-1 Renal transplantation
Divisions:
Page Range: pp. 1235-1249
Journal or Publication Title: Clinical and experimental nephrology
Journal Index: Pubmed
Volume: 23
Number: 10
Identification Number: https://doi.org/10.1007/s10157-019-01765-y
ISSN: 1437-7799 (Electronic) 1342-1751 (Linking)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/2466

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