Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Transdifferentiation of Human Dental Pulp Stem Cells Into Oligoprogenitor Cells

Tue Jun 18 12:21:48 2024

(2017) Transdifferentiation of Human Dental Pulp Stem Cells Into Oligoprogenitor Cells. Basic and Clinical Neuroscience. pp. 387-394. ISSN 2008-126X

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Introduction: The nerve fibers in central nervous system are surrounded by myelin sheet which is formed by oligodendrocytes. Cell therapy based on oligodendrocytes and their precursors transplantation can hold a promising alternative treatment for myelin sheet repair in demyelinating diseases. Methods: Human Dental Pulp Stem Cells (hDPSCs) are noninvasive, autologous and easy available source with multipotency characteristics, so they are in focus of interest in regenerative medicine. In the present study, hDPSCs were differentiated into oligoprogenitor using glial induction media, containing Retinoic Acid (RA), basic Fibroblast Growth Factor (bFGF), Platelet- Derived Growth Factor (PDGF), N2 and B27. The differentiated Oligoprogenitor Cells (OPCs) were evaluated for nestin, Olig2, NG2 and O4 using immunocytochemistry. Also, the expression of nestin, Olig2 and PDGFR-alpha gens (neuroprogenitor and oligoprogenitor markers) were investigated via RT-PCR technique. Results: The results indicate that glial differentiation medium induces the generation of oligoprogenitor cells as revealed via exhibition of specific glial markers, including Olig2, NG2 and O4. The expersion of nestin gene (neuroprogenitor marker) and Olig2 and PDGFR-alpha genes (oligoprogentor markers) were detected in treated hDPSCs at the end of the induction stage. Conclusion: hDPSCs can be induced to transdifferentiate into oligoprogenitor cells and respond to the routinely applied regents for glial differentiation of mesanchymal stem cells. These data suggest the hDPSCs as a valuable source for cell therapy in neurodegenerative diseases.

Item Type: Article
Haratizadeh, S.UNSPECIFIED
Esmaeilnejad-Moghadam, A.UNSPECIFIED
Hamidabadi, H. G.UNSPECIFIED
Keywords: Dental pulp stem cells Regenerative medicine Oligoprogenitor cells Mesenchymal stem cells marrow stromal cells central-nervous-system neuron-like cells in-vitro multiple-sclerosis differentiation transplantation oligodendrocyte remyelination repair Neurosciences & Neurology
Page Range: pp. 387-394
Journal or Publication Title: Basic and Clinical Neuroscience
Journal Index: ISI
Volume: 8
Number: 5
Identification Number:
ISSN: 2008-126X
Depositing User: مهندس مهدی شریفی

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