Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Trehalose Neuroprotective Effects on the Substantia Nigra Dopaminergic Cells by Activating Autophagy and Non-canonical Nrf2 Pathways

Fri May 24 23:05:09 2024

(2019) Trehalose Neuroprotective Effects on the Substantia Nigra Dopaminergic Cells by Activating Autophagy and Non-canonical Nrf2 Pathways. Iranian Journal of Pharmaceutical Research. pp. 1419-1428. ISSN 1735-0328

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Trehalose, as a natural disaccharide, is known as an autophagy inducer. The neuroprotective effects of trehalose in the rat model of Parkinson's disease were the aim of the present study. Parkinson's disease model was induced by injecting 6-hydroxydopamine (6-OHDA) in the striatum of male Wistar rats. Apomorphine-induced behavior and substantia nigra neuronal counts were applied to evaluate the neuroprotective effects of trehalose. The autophagy was studied using the expression of p62 and LC3II/LC3I ratio. In addition, the antioxidant effects of trehalose were assessed by analyzing the levels of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and also glutathione reductase (GR), glutathione peroxidase (GPx) and Catalase (CAT) enzymes. Moreover, the levels of 3, 4-dihydroxyphenylacetic acid (DOPAC) and dopamine (DA) were assessed. The behavioral test showed that trehalose in the treatment group reduced the damage to the substantial nigra dopaminergic neurons, which was characterized by improved motor and reduced rotations in the treatment group as compared with the lesion group. In the histological examinations of the treatment group, trehalose prevented the destruction of dopaminergic neurons. Trehalose treatments increased autophagy (high LC3II/LC3I ratio) and the expression of the p62 protein as well. Through p62-dependent manner, it led to increased nuclear translocation of Nrf2 transcription factor and elevated expression of downstream antioxidant enzymes, such as GR, GPx, and CAT, restoring DA and DOPAC contents of the cells. In the current study, trehalose simultaneously protects substantia nigra dopaminergic cells by activating both non-canonical p62/SQSTM1-Keap1-Nrf2 and autophagy pathways.

Item Type: Article
Abbaszadeh, H. A.UNSPECIFIED
Keywords: Autophagy Non-canonical Nrf2 pathway p62 Parkinson's disease Trehalose selective hippocampal ischemia transcription factor nrf2 parkinsons-disease keap1-nrf2 pathway oxidative stress mouse model stem-cell rat model alpha gene Pharmacology & Pharmacy
Page Range: pp. 1419-1428
Journal or Publication Title: Iranian Journal of Pharmaceutical Research
Journal Index: ISI
Volume: 18
Number: 3
Identification Number:
ISSN: 1735-0328
Depositing User: مهندس مهدی شریفی

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