Abstract

Allium saralicum R.M. Fritsch has been used in Iranian traditional medicine as a remedial supplement for microbial diseases. This paper reports the green synthesis, chemical characterization and antioxidant, cytotoxic, antibacterial and antifungal properties of silver nanoparticles obtained using aqueous extract of A. saralicum leaves. In this synthesis, no surfactants or stabilizers were used. For characterization, UV-visible spectroscopy, transmission electron microscopy, X-ray diffraction, energy-dispersive X-ray spectroscopy and field emission scanning electron microscopy were used. 2,2-Diphenyl-1-picrylhydrazyl was used in experiments to assess the antioxidant potential of the silver nanoparticles, which revealed an impressive prevention in comparison with butylated hydroxytoluene. The synthesized silver nanoparticles at low doses (1-250 mu g dl(-1)) did not show marked cytotoxic activity (against cervical cancer cells (Hela), breast cancer cells (MCF-7) and human embryonic kidney cells (HEK-293)). Agar diffusion tests were applied to determine the antibacterial and antifungal characteristics. Compared with all standard antimicrobials, the silver nanoparticles showed higher antibacterial and antifungal activities (p <= 0.01). Also, the silver nanoparticles inhibited the growth of all bacteria and fungi at concentrations of 31-250 mu g ml(-1), and destroyed them at concentrations of 31-500 mu g ml(-1) (p <= 0.01). Because the silver nanoparticles obtained using aqueous extract of A. saralicum leaves have antioxidant, non-cytotoxic, antifungal and antibacterial potentials, they can be used as a medical supplement or drug.