Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Comparison of transduction efficiency among various cell types by a lentivector containing CMV promoter

(2019) Comparison of transduction efficiency among various cell types by a lentivector containing CMV promoter. Comparative Clinical Pathology. ISSN 16185641 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Due to their unique biological characteristics, mesenchymal stem cells (MSCs) are useful in developing cell-based therapies, tissue regeneration, and many other applications of cellular and molecular genetics. Use of lentiviral system allows the integration of specific nucleic acid sequence into a target cell genome near an active transcription site in such a way that it is stable and heritable. The aim of the present study was to evaluate the gene transduction efficiency on the cells of different species using lentivirus along with cytomegalovirus in a lentiviral shuttle vector. In this study, the third-passage isolated MSCs from four different species, including ovine and goat cardiac fibroblasts, HEK293T, and L929 cell line were transduced by concentrated virus particles. Then, transduction percentage was detected by flow cytometry. The findings showed different transduction efficiencies for various cells of one species or other species, indicating that differentiated and undifferentiated cells had different transduction percentages. Different degrees of transduction observed in differentiated and undifferentiated cells might be the result of various lentiviral receptors on the plasma membrane, site of integration, and activity of CMV promoter. Further, other regulatory factors involved in cell transduction and target gene expression can cause different percentages of transduction. © 2019, Springer-Verlag London Ltd., part of Springer Nature.

Item Type: Article
Keywords: Cell therapy Gene therapy Lentiviral vectors Mesenchymal stem cells Transfection
Journal or Publication Title: Comparative Clinical Pathology
Journal Index: Scopus
Identification Number: https://doi.org/10.1007/s00580-019-02929-8
ISSN: 16185641 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/2336

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