Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Intrathecal transplantation of Wharton's jelly mesenchymal stem cells suppresses the NLRP1 inflammasome in the rat model of spinal cord injury

Sun Oct 2 15:58:30 2022

(2019) Intrathecal transplantation of Wharton's jelly mesenchymal stem cells suppresses the NLRP1 inflammasome in the rat model of spinal cord injury. Journal of chemical neuroanatomy. pp. 1-8. ISSN 1873-6300 (Electronic) 0891-0618 (Linking)

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Official URL: https://www.ncbi.nlm.nih.gov/pubmed/30703433

Abstract

After spinal cord injury (SCI) local inflammation is induced following secretion of interleukin-1beta (IL-1beta) and IL-18. It has been described that the secretion of IL-1beta and IL-18 is mediated by a cytoplasmic multiprotein complex, termed inflammasome. Mesenchymal stem cells (MSCs) have been extensively used for treating inflammatory diseases in which they showed immunomodulation characteristics. We utilized the anti-inflammatory potential of Wharton's jelly mesenchymal stem cells (WJ-MSCs) to target inflammasome complex in rat SCI model. Real time-polymerase chain reaction, western blotting, and ELISA assay were done one week after SCI to measure the expression of the inflammasome components including NLRP1, ASC, and active caspase-1 as well as IL-1beta, IL-18, and tumor necrosis factor-alpha (TNF-alpha). The histologic alteration and hind-limb locomotion were evaluated three weeks after injury by nissl staining and Basso, Beattie, Bresnahan (BBB), respectively. Our results showed that WJ-MSCs transplantation significantly decreased the SCI-induced expression of the evaluated factors in both mRNA and protein levels. In addition, WJ-MSCs significantly increased the number of normal-appearance neurons in the ventral horn of spinal cord. Noteworthy, these effects resulted in a significant improvement of motor function recovery. We conclude that inflammasome inhibition may be one of the mechanisms for the anti-inflammatory effect of MSCs in the SCI.

Item Type: Article
Creators:
CreatorsEmail
Mohamadi, Y.UNSPECIFIED
Noori Moghahi, S. M. H.UNSPECIFIED
Mousavi, M.UNSPECIFIED
Borhani-Haghighi, M.UNSPECIFIED
Abolhassani, F.UNSPECIFIED
Kashani, I. R.UNSPECIFIED
Hassanzadeh, G.UNSPECIFIED
Keywords: Inflammasome Intrathecal Spinal cord injury Transplantation Wharton's jelly mesenchymal stem cells
Divisions:
Page Range: pp. 1-8
Journal or Publication Title: Journal of chemical neuroanatomy
Journal Index: Pubmed
Volume: 97
Identification Number: https://doi.org/10.1016/j.jchemneu.2019.01.011
ISSN: 1873-6300 (Electronic) 0891-0618 (Linking)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/2312

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