(2019) Intrathecal transplantation of Wharton's jelly mesenchymal stem cells suppresses the NLRP1 inflammasome in the rat model of spinal cord injury. Journal of chemical neuroanatomy. pp. 1-8. ISSN 1873-6300 (Electronic) 0891-0618 (Linking)
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Abstract
After spinal cord injury (SCI) local inflammation is induced following secretion of interleukin-1beta (IL-1beta) and IL-18. It has been described that the secretion of IL-1beta and IL-18 is mediated by a cytoplasmic multiprotein complex, termed inflammasome. Mesenchymal stem cells (MSCs) have been extensively used for treating inflammatory diseases in which they showed immunomodulation characteristics. We utilized the anti-inflammatory potential of Wharton's jelly mesenchymal stem cells (WJ-MSCs) to target inflammasome complex in rat SCI model. Real time-polymerase chain reaction, western blotting, and ELISA assay were done one week after SCI to measure the expression of the inflammasome components including NLRP1, ASC, and active caspase-1 as well as IL-1beta, IL-18, and tumor necrosis factor-alpha (TNF-alpha). The histologic alteration and hind-limb locomotion were evaluated three weeks after injury by nissl staining and Basso, Beattie, Bresnahan (BBB), respectively. Our results showed that WJ-MSCs transplantation significantly decreased the SCI-induced expression of the evaluated factors in both mRNA and protein levels. In addition, WJ-MSCs significantly increased the number of normal-appearance neurons in the ventral horn of spinal cord. Noteworthy, these effects resulted in a significant improvement of motor function recovery. We conclude that inflammasome inhibition may be one of the mechanisms for the anti-inflammatory effect of MSCs in the SCI.
Item Type: | Article | ||||||||||||||||
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Keywords: | Inflammasome Intrathecal Spinal cord injury Transplantation Wharton's jelly mesenchymal stem cells | ||||||||||||||||
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Page Range: | pp. 1-8 | ||||||||||||||||
Journal or Publication Title: | Journal of chemical neuroanatomy | ||||||||||||||||
Journal Index: | Pubmed | ||||||||||||||||
Volume: | 97 | ||||||||||||||||
Identification Number: | https://doi.org/10.1016/j.jchemneu.2019.01.011 | ||||||||||||||||
ISSN: | 1873-6300 (Electronic) 0891-0618 (Linking) | ||||||||||||||||
Depositing User: | مهندس مهدی شریفی | ||||||||||||||||
URI: | http://eprints.medilam.ac.ir/id/eprint/2312 |
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