Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

In utero transplantation of neural stem cells ameliorates maternal inflammation-induced prenatal white matter injury

Sun May 19 23:45:02 2024

(2019) In utero transplantation of neural stem cells ameliorates maternal inflammation-induced prenatal white matter injury. Journal of cellular biochemistry. ISSN 1097-4644 (Electronic) 0730-2312 (Linking)

Full text not available from this repository.

Official URL:


Prenatal white matter injury is a serious problem due to maternal inflammation leading to postnatal disabilities. In this study, we used the periventricular leukomalacia (PVL) model as a common prenatal white matter injury by maternal administration of lipopolysaccharide (LPS). Neural stem cells (NSCs) have shown therapeutic ability in neurological disorders through a different mechanism such as immunomodulation. Here, we studied the preventive potential of NSCs following in utero transplantation into the embryonic lateral ventricle in an LPS-induced white matter injury model. Pregnant animals were divided into three groups and received phosphate buffered saline, LPS, or LPS + NSCs. The brains of offspring were obtained and evaluated by real-time polymerase chain reaction (PCR), immunohistochemy, enzyme-linked immunosorbent assay (ELISA), terminal deoxynucleotidyl transferase-mediated biotinylated-dUTP nick-end labeling (TUNEL), and caspase-3 activity assay. The LPS-induced maternal inflammation degenerated the myelin sheath in the offspring periventricular region which was associated with an increased microglial number, oligodendrocytes degeneration, proinflammatory cytokine secretion, and cell apoptosis. The transplanted NSCs homed into the brain and ameliorated the evaluated parameters. The expression of proinflammatory cytokines interleukin-1beta (IL-1beta), IL-6, and tumor necrosis factor-alpha (TNF-alpha), cell apoptosis and caspase-3 activity were inhibited by NSCs. In addition, Olig2 and myelin basic protein immunohistochemy staining showed that prenatal NSCs transplantation augmented the myelination in the periventricular white matter of offspring. In conclusion, we think that prenatal therapeutic strategies, such as in utero NSCs transplantation, may prevent prenatal white matter injury after birth.

Item Type: Article
Borhani-Haghighi, M.UNSPECIFIED
Keywords: inflammation lipopolysaccharide neural stem cell white matter injury
Journal or Publication Title: Journal of cellular biochemistry
Journal Index: Pubmed
Identification Number:
ISSN: 1097-4644 (Electronic) 0730-2312 (Linking)
Depositing User: مهندس مهدی شریفی

Actions (login required)

View Item View Item