Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Toxicity study of silver nanoparticles synthesized from aqueous flower extract of Scrophularia striata on MCF-7 human breast cancer cell line

Thu May 23 04:05:45 2024

(2019) Toxicity study of silver nanoparticles synthesized from aqueous flower extract of Scrophularia striata on MCF-7 human breast cancer cell line. Pharmacognosy Magazine. pp. 66-72. ISSN 0973-1296

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Background: Silver nanoparticles (AgNPs) have been used as an antimicrobial and disinfectant agent. Nevertheless, there is limited data about antitumor potential. This study has focused on investigating cytotoxic effects of AgNPs from Scrophularia striata flower extract on MCF-7 breast cancer cells and its mechanism of action. Materials and Methods: Thus, a green method was created for the synthesis of AgNPs using an aqueous extract of S. striata flower. Synthesis of AgNPs was described by different analytical techniques including ultraviolet-visible spectrophotometer, field-emission scanning electron microscopy, X-ray diffraction, and Fourier transforms infrared spectroscopy. Cell viability was determined by the 3-4, 5-dimethylthiazol-2-yl-a 2,5-diphenyltetrazolium bromide assay. Reactive oxygen species (ROS) formation was measured using probe 2', 7'-dichlorofluorescein diacetate and intracellular calcium (Cai(2+)) was evaluated with probe flu3-AM. Cells were treated with different concentrations of AgNPs (1, 3, 6, 10, 15, 25, 50, and 100 mg/mL). Results: The results showed that AgNPs hindered cell growth in a dose-dependent manner. AgNPs appeared to have dose-dependent cytotoxicity against MCF-7 cells through activation of the ROS generation and an increase in the intracellular Cai(2+) (IC50 52 3.14). Conclusion: In conclusion, the results of this preliminary study demonstrated that AgNPs from S. striata flower extract may be a potential therapeutic agent for human breast cancer treatment. Abbreviations used: S. striata: Scrophularia striata; FESEM: Field-emission scanning electron microscopy; XRD: X-ray diffraction; FTIR: Fourier transforms infrared spectroscopy; MTT: 3-4, 5-dimethylthiazol-2-yl-a 2,5-diphenyltetrazolium bromide; ROS: Reactive oxygen species; DCF: 2', 7'-dichlorofluorescein diacetate; Cai(2+): Intracellular calcium.

Item Type: Article
Keywords: Biosynthesis cytotoxicity MCF-7 cell line Scrophularia striata silver nanoparticles green synthesis plant cytotoxicity antibacterial genotoxicity antioxidant carcinoma delivery size Pharmacology & Pharmacy
Page Range: pp. 66-72
Journal or Publication Title: Pharmacognosy Magazine
Journal Index: ISI
Volume: 15
Number: 60
Identification Number:₇₈₁₈
ISSN: 0973-1296
Depositing User: مهندس مهدی شریفی

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