Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Chemical characterization and antioxidant, cytotoxic, antibacterial, and antifungal properties of ethanolic extract of Allium Saralicum RM Fritsch leaves rich in linolenic acid, methyl ester

Sat Oct 1 17:30:31 2022

(2019) Chemical characterization and antioxidant, cytotoxic, antibacterial, and antifungal properties of ethanolic extract of Allium Saralicum RM Fritsch leaves rich in linolenic acid, methyl ester. Journal of Photochemistry and Photobiology B-Biology. pp. 103-112. ISSN 1011-1344

Full text not available from this repository.

Official URL: http://apps.webofknowledge.com/InboundService.do?F...

Abstract

The purpose of the recent research was to assess the chemical characterization and antioxidant, cytotoxic, antibacterial, and antifungal effects of Allium Saralicum R.M. Fritsch leaves. After identification of the plant, its ethanolic extract was obtained using Soxhlet extractor without leaving any chemicals in it. Gas chromatography-mass spectrometry (GC/MS) was performed to detect the percentage, retention index, and time of A. Saralicum compounds. Agar diffusion tests were applied to determine the antibacterial and antifungal characteristics. In agar disk diffusion test, dimethyl sulfoxide (DMSO) was used as negative control, while antibacterial (Difloxacin, Chloramphenicol, Streptomycin, Gentamicin, Oxytetracycline, Ampicillin, and Amikacin) and antifungal (Fluconazole, Itraconazole, Miconazole, Amphotericin B, and Nystatin) antibiotics were used as positive controls. Macro broth tube test was run to determine Minimum Inhibitory Concentration (MIC). The findings indicated that linolenic acid, methyl ester was the most frequent constituent found in A. Saralicum. Indeed, A. Saralicum showed higher antibacterial and antifungal properties than all standard antibiotics (p <= 01). Also, A. Saralicum prevented the growth of all bacteria and fungi at 15-125 mg/mL concentrations and destroyed them at 15-250 mg/mL concentrations (p <= 01). DPPH free radical scavenging test was carried out to examine the antioxidant effect, which indicated similar antioxidant activity with butylated hydroxy toluene (BHT) as a positive control. The synthesized ethanolic extract had great cell viability dose-dependently and demonstrated this method was nontoxic for synthesizing A. Saralicum. In conclusion, the findings showed the useful antioxidant, non-cytotoxic, antibacterial, and antifungal effects of A. Saralicum ethanolic extract.

Item Type: Article
Creators:
CreatorsEmail
Jalalvand, A. R.UNSPECIFIED
Zhaleh, M.UNSPECIFIED
Goorani, S.UNSPECIFIED
Zangeneh, M. M.UNSPECIFIED
Seydi, N.UNSPECIFIED
Zangeneh, A.UNSPECIFIED
Moradi, R.UNSPECIFIED
Keywords: Allium Saralicum RM Fritsch Chemical composition Antioxidant Cytotoxicity Antibacterial Antifungal essential oil root Biochemistry & Molecular Biology Biophysics
Divisions:
Page Range: pp. 103-112
Journal or Publication Title: Journal of Photochemistry and Photobiology B-Biology
Journal Index: ISI
Volume: 192
Identification Number: https://doi.org/10.1016/j.jphotobiol.2019.01.017
ISSN: 1011-1344
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/2258

Actions (login required)

View Item View Item