Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

DNA vaccination with a plasmid encoding LACK-TSA fusion against Leishmania major infection in BALB/c mice

Sat Jul 20 20:37:10 2024

(2017) DNA vaccination with a plasmid encoding LACK-TSA fusion against Leishmania major infection in BALB/c mice. Malaysian Journal of Pathology. pp. 267-275. ISSN 0126-8635

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Vaccination would be the most important strategy for the prevention and elimination of leishmaniasis. The aim of the present study was to compare the immune responses induced following DNA vaccination with LACK (Leishmania analogue of the receptor kinase C), TSA (Thiol-specific-antioxidant) genes alone or LACK-TSA fusion against cutaneous leishmaniasis (CL). Cellular and humoral immune responses were evaluated before and after challenge with Leishmania major (L. major). In addition, the mean lesion size was also measured from 3th week post-infection. All immunized mice showed a partial immunity characterized by higher interferon (IFN)-gamma and Immunoglobulin G (IgG2a) levels compared to control groups (p< 0.05). IFN-gamma/Interleukin (IL)-4 and IgG2a/IgG1 ratios demonstrated the highest IFN-gamma and IgG2a levels in the group receiving LACK-TSA fusion. Mean lesion sizes reduced significantly in all immunized mice compared with control groups at 7th week post-infection (p< 0.05). In addition, there was a significant reduction in mean lesion size of LACK-TSA and TSA groups than LACK group after challenge (p< 0.05). In the present study, DNA immunization promoted Th1 immune response and confirmed the previous observations on immunogenicity of LACK and TSA antigens against CL. Furthermore, this study demonstrated that a bivalent vaccine can induce stronger immune responses and protection against infectious challenge with L. major.

Item Type: Article
Ghaffarifar, F.UNSPECIFIED
Keywords: Leishmania vaccine LACK TSA fusion murine cutaneous leishmaniasis cd4(+) t-cells visceral leishmaniasis polyprotein vaccine immune-responses recombinant antigens drug targets cpg DNA candidates protection Pathology
Page Range: pp. 267-275
Journal or Publication Title: Malaysian Journal of Pathology
Journal Index: ISI
Volume: 39
Number: 3
ISSN: 0126-8635
Depositing User: مهندس مهدی شریفی

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