Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Thermodynamic domain analysis of fresh and incubated human apotransferrin

(2002) Thermodynamic domain analysis of fresh and incubated human apotransferrin. Thermochimica Acta. pp. 103-108. ISSN 00406031 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

The thermal denaturation of human apotransferrin was studied by a differential scanning calorimeter (DSC) in 100 mM Hepes buffer at pH 7.0. Deconvolution analysis of excess molar heat capacity was adjusted by four transitions of which two transitions were linked to N-domain and the remaining two were connected to C-domain. The same experiment was carried out for the incubated sample for a period of 24 h at 27°C. The deconvolution subpeaks exhibited five transitions. The free energy, ΔG°H2O, in the absence of sodium n-dodecyl sulphate (SDS), as a criterion of stability measurement, was obtained based on Pace theory on UV spectrophotometry. The result indicates that incubated sample was stabilized by 14 kJ mol-1 relative to the fresh sample. The circular dichroism (CD) study confirms the presence of additional secondary structures for the incubated sample compared with the fresh one because of Hepes molecule interaction with protein domains. © 2002 Elsevier Science B.V. All rights reserved.

Item Type: Article
Keywords: Additional subpeak Deconvolution analysis Differential scanning calorimetry (DSC) Hepes buffer Incubated apotransferrin apotransferrin dodecyl sulfate article circular dichroism differential scanning calorimetry molecular stability phase transition protein domain protein interaction thermodynamics ultraviolet spectroscopy
Page Range: pp. 103-108
Journal or Publication Title: Thermochimica Acta
Journal Index: Scopus
Volume: 383
Number: 1-2
Identification Number: https://doi.org/10.1016/S0040-6031(01)00684-0
ISSN: 00406031 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1696

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