Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Binding properties and conformational change of human growth hormone upon interaction with Fe3+

Sat Aug 13 17:18:11 2022

(2005) Binding properties and conformational change of human growth hormone upon interaction with Fe3+. Thermochimica Acta. pp. 178-183. ISSN 00406031 (ISSN)

Full text not available from this repository.

Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Binding properties and conformational change of human growth hormone (hGH) upon interaction with Fe3+ were investigated at 27 °C in NaCl solution, 50 mM, by calorimetry and spectroscopy. UV spectroscopy indicates that thermal denaturation of hGH is an irreversible process and is accompanied by aggregation. At an optimum concentration of iron thermal denaturation of hGH becomes reversible. Results from equilibrium dialysis and isothermal titration calorimetry indicate a set of four binding sites on hGH for Fe3+. Interaction of three iron ions with hGH prevents irreversibility and aggregation. Differential scanning calorimetry confirms the UV spectroscopic finding. Domain analysis by DSC shows that in the presence of iron, there are at least two main transitions corresponding with the two groups of helices. Deconvolution of the main transitions provides two sub-transitions each, the first pair is similar, but the second pair is considerably different in the enthalpy change of unfolding. Interaction of iron ions with hGH prevents aggregation by an effect on the hydrophobicity and provides information about its structure and thermal denaturation. © 2005 Elsevier B.V. All rights reserved.

Item Type: Article
Creators:
CreatorsEmail
Atri, M. S.UNSPECIFIED
Saboury, A. A.UNSPECIFIED
Rezaei-Tavirani, M.UNSPECIFIED
Sanati, M. H.UNSPECIFIED
Moosavi-Movahedi, A. A.UNSPECIFIED
Sadeghi, M.UNSPECIFIED
Mansuri-Torshizi, H.UNSPECIFIED
Khodabandeh, M.UNSPECIFIED
Keywords: Conformational change Differential scanning calorimetry (DSC) Fe3+ Human growth hormone (hGH) Isothermal titration calorimetry (ITC) Metal binding Calorimetry Differential scanning calorimetry Hydrophobicity Iron Sodium chloride Titration Ultraviolet spectroscopy Conformational changes Human growth hormone Hormones
Divisions:
Page Range: pp. 178-183
Journal or Publication Title: Thermochimica Acta
Journal Index: Scopus
Volume: 438
Number: 1-2
Identification Number: https://doi.org/10.1016/j.tca.2005.08.033
ISSN: 00406031 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1690

Actions (login required)

View Item View Item