Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Comparison of different laboratory methods for diagnosis of Helicobacter pylori

Thu Oct 6 04:45:44 2022

(2006) Comparison of different laboratory methods for diagnosis of Helicobacter pylori. Journal of Biological Sciences. pp. 1146-1149. ISSN 17273048 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

The aim of the present study was to compare four laboratory methods for diagnosis of Helicobacter pylori. 101 sets of four antral biopsies were collected from 101 patients, one of the biopsies in each set were tested by rapid urease test, other three biopsies were used for culture, direct staining and histopathology, respectively. By culture method in 62 cases (61.3), Helicobacterpylori were isolated. Among several primary media that tested in this study, Brucella agar supplemented with 10 whole sheep blood supported relatively good growth of H. pylori. In present study 65 cases (64.3) were positive by rapid urease test. Of 101 biopsy specimens in 68 cases (67.3) were obtained positive result by histopathology method (Gimsa Staining). Sensitivity and specificity of direct staining test were 89.7 and 96.9, respectively. We found histopathology method was the best method for diagnosis of H. pylori and it can be selected as gold standard in detection of Helicobacter pylori. © 2006 Asian Network for Scientific Information.

Item Type: Article
Creators:
CreatorsEmail
Sadeghifard, N.UNSPECIFIED
Aslani, M. M.UNSPECIFIED
Ghasemi, A.UNSPECIFIED
Keywords: Helicobacter pylori Histopathology Rapid urease test article bacterial growth bacterium detection bacterium isolate bacterium isolation chronic gastritis culture medium diagnostic accuracy diagnostic value embryo gold standard Helicobacter infection human human tissue intermethod comparison laboratory diagnosis major clinical study nonhuman sensitivity and specificity staining stomach antrum stomach biopsy tissue culture Brucella Ovis aries
Divisions:
Page Range: pp. 1146-1149
Journal or Publication Title: Journal of Biological Sciences
Journal Index: Scopus
Volume: 6
Number: 6
Identification Number: https://doi.org/10.3923/jbs.2006.1146.1149
ISSN: 17273048 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1689

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