Repository of Research and Investigative Information

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Ilam University of Medical Sciences

The mosaic nature of intergenic 16S-23S rRNA spacer regions suggests rRNA operon copy number variation in Clostridium difficile strains

Mon Jul 22 14:58:34 2024

(2006) The mosaic nature of intergenic 16S-23S rRNA spacer regions suggests rRNA operon copy number variation in Clostridium difficile strains. Applied and Environmental Microbiology. pp. 7311-7323. ISSN 00992240 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Clostridium difficile is a major spore-forming environmental pathogen that causes serious health problems in patients undergoing antibiotic therapy. Consequently, reliable and sensitive methods for typing individual strains are required for epidemiological and environmental studies. Ribotyping is generally considered the best method, but it fails to account for sequence diversity which might exist in intergenic 16S-23S rRNA spacer regions (ISRs) within and among strains of this organism. Therefore, this study was undertaken to compare the sequence of each individual ISR in five strains of C. difficile to explore the extent of this diversity and see whether such information might provide the basis for more sensitive and discriminatory strain typing methods. After targeted PCR amplification, cloning, and sequencing, the diversity of the ISRs was used as a measure of rRNA operon copy number. In C. difficile strains 630, ATCC 43593, A, and B, 11, 11, 7, and 8 ISR length variants, respectively, were found (containing different combinations of sequence groups i to xiii), suggesting 11, 11, 7, and 8 rrn copies in the respective strains. Many ISRs of the same length differed markedly in their sequences, and some of these were restricted in occurrence to a single strain. Most of these ISRs did not contain any tRNA genes, and only single copies of the tRNAAla gene were found in those that did. The presence of ISR sequence groups (i to xiii) varied between strains, with some found in one, two, three, four, or all five strains. We conclude that the intergenic 16S-23S rRNA spacer regions showed a high degree of diversity, not only among the rrn operons in different strains and different rrn copies in a single strain but also among ISRs of the same length. It appears that C. difficile ISRs vary more at the inter- and intragenic levels than those of other species as determined by empirical comparison of sequences. The precise characterization of these sequences has demonstrated a high level of mosaic sequence block rearrangements that are present or absent in multiple strain-variable rrn copies within and between five different strains of C. difficile. Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Item Type: Article
Creators:
CreatorsEmail
Sadeghifard, N.UNSPECIFIED
Gürtler, V.UNSPECIFIED
Beer, M.UNSPECIFIED
Seviour, R. J.UNSPECIFIED
Keywords: Antibiotics Biodiversity Drug therapy Environmental impact Genes Pathology RNA Antibiotic therapy Clostridium difficile Intergenic 16S-23S rRNA spacer regions (ISR) Mosaic Bacteria antibiotic agent bacterial DNA bacterial RNA ribosome RNA RNA 16S RNA 23S spacer DNA transfer RNA bacterial disease bacterium bioassay genetic structure genetic variation genotype article bacterial genetics bacterial strain bacterium identification gene amplification gene sequence genetic variability molecular cloning nonhuman nucleotide sequence operon polymerase chain reaction reliability ribotyping sensitivity and specificity Bacterial Typing Techniques Base Sequence DNA, Ribosomal Spacer Gene Dosage Humans Molecular Sequence Data Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S RNA, Ribosomal, 23S rRNA Operon Sequence Analysis, DNA Species Specificity Variation (Genetics)
Divisions:
Page Range: pp. 7311-7323
Journal or Publication Title: Applied and Environmental Microbiology
Journal Index: Scopus
Volume: 72
Number: 11
Identification Number: https://doi.org/10.1128/AEM.01179-06
ISSN: 00992240 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1683

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