Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Binding properties and structural changes of human growth hormone upon interaction with cobalt ion

Wed Aug 17 23:26:17 2022

(2007) Binding properties and structural changes of human growth hormone upon interaction with cobalt ion. Journal of Thermal Analysis and Calorimetry. pp. 921-927. ISSN 13886150 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Binding properties and structural changes of human growth hormone (hGH) due to the interaction by cobalt ion (Co2+) were done at 27°C in NaCl solution, 50 mM, using different techniques of UV-Vis spectroscopy, circular dichroism (CD), isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC) techniques. There is a set of three identical and non-interacting binding sites for cobalt ions. The intrinsic association equilibrium constant and the molar enthalpy of binding obtained by ITC are 0.80 mM-1 and -16.70 kJ mol-1, respectively. The intrinsic association equilibrium constant obtained by a standard isothermal titration UV-Vis spectrophotometry method is also 0.79 mM-1, which is in good agreement with the value obtained from ITC. The Gibbs free energy and entropy changes due to the binding of cobalt ion on hGH are -16.67 kJ mol-1 and -0.1 J K-1 mol-1, respectively. Energetic domains analysis by DSC shows that phase transition of hGH in the presence of cobalt occurs at one main transition. Deconvolution of the main transition provides two sub-transitions with different values of the melting point and enthalpy of unfolding (33°C and 164 kJ mol-1 for the first and 49°C and 177 kJ mol-1 for the second, respectively). Interaction of cobalt ions with hGH prevents aggregation by an affect on the hydrophobicity of the protein macromolecule and provide useful information about its structure due to becoming reversible of protein thermal denaturation. © 2007 Springer Science+Business Media LLC.

Item Type: Article
Creators:
CreatorsEmail
Saboury, A. A.UNSPECIFIED
Ghourchaei, H.UNSPECIFIED
Sanati, M. H.UNSPECIFIED
Atri, M. S.UNSPECIFIED
Rezaei-Tawirani, M.UNSPECIFIED
Hakimelahi, G. H.UNSPECIFIED
Keywords: Circular dichroism Cobalt Human growth hormone Protein stability Titration calorimetry Binding energy Dichroism Differential scanning calorimetry Growth kinetics Human engineering Molecular interactions Titration Binding properties Hormones
Divisions:
Page Range: pp. 921-927
Journal or Publication Title: Journal of Thermal Analysis and Calorimetry
Journal Index: Scopus
Volume: 89
Number: 3
Identification Number: https://doi.org/10.1007/s10973-006-7533-2
ISSN: 13886150 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1671

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